ArticleJournal of visualized experiments : JoVE2016
Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA.
Article in Journal of visualized experiments : JoVE, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Molecular exploration of mosquitoes and their vector-borne pathogens in urban water environments in Benin.Scientific reports · 2026Article
- Influence of Deadwood, Tree-Related Microhabitats, and Forest Structural Features on Saproxylic Arthropod Diversity.Ecology and evolution · 2026Article
- Large-scale environmental DNA survey reveals niche axes of a regional coastal fish community.Scientific reports · 2026Article
- Environmental Filtering Weakens with Trophic Level in Urban Coastal Ecosystems.Environmental science & technology · 2026Article
- QuickConc: A Rapid, Efficient, and Power-Free eDNA Concentration Method With Cationic-Assisted Capture.Ecology and evolution · 2025Article
- A molecular specimen bank for contemporary and future study captures landscape-scale biodiversity baselines before Klamath River dam removal.Scientific reports · 2025Article
- Fish diversity assessment through conventional morphological identification and recent advances in Saudi Arabia: A review.Veterinary world · 2024Review
- Comparison and Optimization of Simple DNA Extraction Methods for LAMP-Based Point-of-Care Applications Employing Submillimeter Skin Biopsies.ACS omega · 2024Article
- Article
- Vertical eDNA distribution of cold-water fishes in response to environmental variables in stratified lake.Ecology and evolution · 2024Article
- Article
- Benchmarking DNA isolation methods for marine metagenomics.Scientific reports · 2023Article
- Collection of environmental DNA from stemflow for monitoring arboreal biodiversity: Preliminary validation using lichens.MethodsX · 2023Article
- Temperature sensitivity of the interspecific interaction strength of coastal marine fish communities.eLife · 2023Article
- Non-destructive collection and metabarcoding of arthropod environmental DNA remained on a terrestrial plant.Scientific reports · 2023Article
- Improvement of recovery yield of macro-organismal environmental DNA from seawater samples.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2023Article
- Article
- Comparative environmental RNA and DNA metabarcoding analysis of river algae and arthropods for ecological surveys and water quality assessment.Scientific reports · 2022Article
- Effects of forest cover on richness of threatened fish species in Japan.Conservation biology : the journal of the Society for Conservation Biology · 2022Article
- Phylogenetic relationship and characterization of the complete mitochondrial genome sequence ofMitochondrial DNA. Part B, Resources · 2022Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent studies demonstrated the use of environmental DNA (eDNA) from fishes to be appropriate as a non-invasive monitoring tool. Most of these studies employed disk fiber filters to collect eDNA from water samples, although a number of microbial studies in aquatic environments have employed filter cartridges, because the cartridge has the advantage of accommodating large water volumes and of overall ease of use. Here we provide a protocol for filtration of water samples using the filter cartridge and extraction of eDNA from the filter without having to cut open the housing. The main portions of this protocol consists of 1) filtration of water samples (water volumes ≤4 L or >4 L); (2) extraction of DNA on the filter using a roller shaker placed in a preheated incubator; and (3) purification of DNA using a commercial kit. With the use of this and previously-used protocols, we perform metabarcoding analysis of eDNA taken from a huge aquarium tank (7,500 m
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