ArticleJournal of visualized experiments : JoVE2016
The Mouse Isolated Perfused Kidney Technique.
Article in Journal of visualized experiments : JoVE, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- Gut and Glomerular Barriers Determine Nanoplastic Fate and Systemic Impact.ACS environmental Au · 2026Article
- MUC16-dependent Renal Vascular Adhesion of Candida Promotes Tissue Invasion and Predicts Clinical Outcome in Candidemia.Research square · 2026Article
- Isolated perfusion of pig kidney segments to study renal function: a translational model for human kidneys.Laboratory animals · 2026Article
- Label-free viability assessment ofBiomedical optics express · 2026Article
- Metabolite-enhanced normothermic machine perfusion improves kidney transplant viability.JCI insight · 2025Article
- Electrophysiology Models for End-Stage Renal Disease Maladaptations That Promote Asystole.Reviews in cardiovascular medicine · 2025Review
- Functional Microendoscopy Reveals Calcium Responses of Single Cells in Tracheal Tuft Cells and Kidney Podocytes.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- The ex vivo perfused mouse adrenal gland-a new model to study aldosterone secretion.Pflugers Archiv : European journal of physiology · 2024Article
- Terabyte-scale supervised 3D training and benchmarking dataset of the mouse kidney.Scientific data · 2023Article
- Single-cell transcriptomics reveals a mechanosensitive injury signaling pathway in early diabetic nephropathy.Genome medicine · 2023Article
- Mapping single-nephron filtration in the isolated, perfused rat kidney using magnetic resonance imaging.American journal of physiology. Renal physiology · 2022Article
- A high-throughput drug discovery pipeline to optimize kidney normothermic machine perfusion.Frontiers in physiology · 2022Article
- Effectiveness and safety of retrograde intrarenal surgery (RIRS) vs. percutaneous nephrolithotomy (PCNL) in the treatment of isolated kidney stones.American journal of translational research · 2022Article
- Creation of an ex-vivo bovine kidney flow model for testing embolic agents: work in progress.CVIR endovascular · 2021Article
- Lack of connexin 40 decreases the calcium sensitivity of renin-secreting juxtaglomerular cells.Pflugers Archiv : European journal of physiology · 2018Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mouse isolated perfused kidney (MIPK) is a technique for keeping a mouse kidney under ex vivo conditions perfused and functional for 1 hr. This is a prerequisite for studying the physiology of the isolated organ and for many innovative applications that may be possible in the future, including perfusion decellularization for kidney bioengineering or the administration of anti-rejection or genome-editing drugs in high doses to prime the kidney for transplantation. During the time of the perfusion, the kidney can be manipulated, renal function can be assessed, and various pharmaceuticals administered. After the procedure, the kidney can be transplanted or processed for molecular biology, biochemical analysis, or microscopy. This paper describes the perfusate and the surgical technique needed for the ex vivo perfusion of mouse kidneys. Details of the perfusion apparatus are given and data are presented showing the viability of the kidney's preparation: renal blood flow, vascular resistance, and urine data as functional, transmission electron micrographs of different nephron segments as morphological readouts, and western blots of transport proteins of different nephron segments as molecular readout.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.