ArticleScientific reports2016
Improved bi-allelic modification of a transcriptionally silent locus in patient-derived iPSC by Cas9 nickase.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed.
- Leveraging a self-cleaving peptide for tailored control in proximity labeling proteomics.Cell reports methods · 2024Article
- Recent Advances in CRISPR/Cas9 Delivery Approaches for Therapeutic Gene Editing of Stem Cells.Stem cell reviews and reports · 2023Review
- Oncogene-like addiction to aneuploidy in human cancers.Science (New York, N.Y.) · 2023Article
- Transgene Expression and Transposition Efficiency of Two-Component Sleeping Beauty Transposon Vector Systems Utilizing Plasmid or mRNA Encoding the Transposase.Molecular biotechnology · 2023Article
- Enrichment strategies to enhance genome editing.Journal of biomedical science · 2023Review
- Oncogene-like addiction to aneuploidy in human cancers.bioRxiv : the preprint server for biology · 2023Article
- CRISPR/Cas9 Genome Editing in LGMD2A/R1 Patient-Derived Induced Pluripotent Stem and Skeletal Muscle Progenitor Cells.Stem cells international · 2023Article
- A high-efficiency and versatile CRISPR/Cas9-mediated HDR-based biallelic editing system.Journal of Zhejiang University. Science. B · 2022Article
- A selectable all-in-one CRISPR prime editing piggyBac transposon allows for highly efficient gene editing in human cell lines.Scientific reports · 2021Article
- Use of single guided Cas9 nickase to facilitate precise and efficient genome editing in human iPSCs.Scientific reports · 2021Article
- A Cas9-mediated adenosine transient reporter enables enrichment of ABE-targeted cells.BMC biology · 2020Article
- Modeling Psychiatric Disorder Biology with Stem Cells.Current psychiatry reports · 2020Review
- A combined in silico and in vitro study on mouse Serpina1a antitrypsin-deficiency mutants.Scientific reports · 2019Article
- Developing precision medicine using scarless genome editing of human pluripotent stem cells.Drug discovery today. Technologies · 2018Review
- Scarless Genome Editing of Human Pluripotent Stem Cells via Transient Puromycin Selection.Stem cell reports · 2018Article
- FACS-Assisted CRISPR-Cas9 Genome Editing Facilitates Parkinson's Disease Modeling.Stem cell reports · 2017Article
- CRISPR-Cas orthologues and variants: optimizing the repertoire, specificity and delivery of genome engineering tools.Mammalian genome : official journal of the International Mammalian Genome Society · 2017Review
- Editing the genome of hiPSC with CRISPR/Cas9: disease models.Mammalian genome : official journal of the International Mammalian Genome Society · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
19 authors.
Funding
Abstract
Homology directed repair (HDR)-based genome editing via selectable long flanking arm donors can be hampered by local transgene silencing at transcriptionally silent loci. Here, we report efficient bi-allelic modification of a silent locus in patient-derived hiPSC by using Cas9 nickase and a silencing-resistant donor construct that contains an excisable selection/counter-selection cassette. To identify the most active single guide RNA (sgRNA)/nickase combinations, we employed a lentiviral vector-based reporter assay to determine the HDR efficiencies in cella. Next, we used the most efficient pair of sgRNAs for targeted integration of an improved, silencing-resistant plasmid donor harboring a piggyBac-flanked puroΔtk cassette. Moreover, we took advantage of a dual-fluorescence selection strategy for bi-allelic targeting and achieved 100% counter-selection efficiency after bi-allelic excision of the selection/counter-selection cassette. Together, we present an improved system for efficient bi-allelic modification of transcriptionally silent loci in human pluripotent stem cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.