ArticlePlant molecular biology2017
Over-expression of miR158 causes pollen abortion in Brassica campestris ssp. chinensis.
Article in Plant molecular biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 30 citations in OpenAlex.
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- Bioinformatics Analysis Reveals PPR Genes Modulation by Ahyp-miR0005 Under Abiotic Stress Across Diverse Plant Species.Plants (Basel, Switzerland) · 2025Article
- Artificial miRNAs and target-mimics as potential tools for crop improvement.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
- miRNA Sequencing Analysis in Maize Roots Treated with Neutral and Alkaline Salts.Current issues in molecular biology · 2024Article
- Genomic survey of high-throughput RNA-Seq data implicates involvement of long intergenic non-coding RNAs (lincRNAs) in cytoplasmic male-sterility and fertility restoration in pigeon pea.Genes & genomics · 2023Article
- Identification ofPlants (Basel, Switzerland) · 2022Article
- Transcript Profiling Analysis and ncRNAs' Identification of Male-Sterile Systems ofFrontiers in plant science · 2022Article
- Identification of miRNAs and their target genes in genic male sterility lines in Brassica napus by small RNA sequencing.BMC plant biology · 2021Article
- Circular RNAs Repertoire and Expression Profile duringInternational journal of molecular sciences · 2021Article
- Global Analysis of the Genetic Variations in miRNA-Targeted Sites and Their Correlations With Agronomic Traits in Rapeseed.Frontiers in genetics · 2021Article
- Utilization of microRNAs and their regulatory functions for improving biotic stress tolerance in tea plant [RNA biology · 2020Article
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- MicroRNAs and new biotechnological tools for its modulation and improving stress tolerance in plants.Plant biotechnology journal · 2019Review
- Non-coding RNAs and plant male sterility: current knowledge and future prospects.Plant cell reports · 2018Review
- Small RNA Sequencing Reveals Differential miRNA Expression in the Early Development of Broccoli (Frontiers in plant science · 2017Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageWe identified and cloned the two precursors of miR158 and its target gene in Brassica campestris ssp. chinensis, which both had high relative expression in the inflorescences. Further study revealed that over-expression of miR158 caused reduced pollen varbility, which was caused by the degradation of pollen contents from the binucleate microspore stage. These results first suggest the role of miR158 in pollen development of Brassica campestris ssp. chinensis. MicroRNAs (miRNAs) play crucial roles in many important growth and development processes both in plants and animals by regulating the expression of their target genes via mRNA cleavage or translational repression. In this study, miR158, a Brassicaceae specific miRNA, was functionally characterized with regard to its role in pollen development of non-heading Chinese cabbage (Brassica campestris ssp. chinensis). Two family members of miR158 in B. campestris, namely bra-miR158a1 and bra-miR158a2, and their target gene bra027656, which encodes a pentatricopeptide repeat (PPR) containing protein, were identified. Then, qRT-PCR analysis and GUS-reporter system revealed that both bra-miR158 and its target gene had relatively high expression levels in the inflorescences. Further study revealed that over-expression of miR158 caused reduced pollen varbility and pollen germination ratio, and the degradation of pollen contents from the binucleate microspore stage was also found in those deformed pollen grains, which led to pollen shrinking and collapse in later pollen development stage. These results first shed light on the importance of miR158 in pollen development of Brassica campestris ssp. chinensis.
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