ArticleGenomics data2017
Traditional
Article in Genomics data, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed.
- The skeletal transcriptional response to mechanical load varies with age and tissue compartment in female mice.JBMR plus · 2025Article
- Bioenergetic programs of cancellous and cortical bone are distinct and differ with age and mechanical loading.Scientific reports · 2025Article
- Optimizing Cost-Effective gene expression phenotyping approaches in cattle using 3' mRNA sequencing.BMC genomics · 2025Article
- Strong correlation of gene counts and differentially expressed genes between a 3' RNA-Seq and an RNA hybridization platform in transcriptome analyses from canine archival tissues.Frontiers in veterinary science · 2025Article
- The hidden RNA viruses inMicrobial genomics · 2024Article
- The cacao gene atlas: a transcriptome developmental atlas reveals highly tissue-specific and dynamically-regulated gene networks in Theobroma cacao L.BMC plant biology · 2024Article
- De novo assembly of transcriptomes and differential gene expression analysis using short-read data from emerging model organisms - a brief guide.Frontiers in zoology · 2024Review
- PTH treatment before cyclic joint loading improves cartilage health and attenuates load-induced osteoarthritis development in mice.Science advances · 2024Article
- Grapevine scion gene expression is driven by rootstock and environment interaction.BMC plant biology · 2023Article
- Resting cytosol Ca2+ level maintained by Ca2+ pumps affects environmental responses in Arabidopsis.Plant physiology · 2023Article
- 3' RNA-seq is superior to standard RNA-seq in cases of sparse data but inferior at identifying toxicity pathways in a model organism.Frontiers in bioinformatics · 2023Article
- Patterns of pan-genome occupancy and gene coexpression under water-deficit in Brachypodium distachyon.Molecular ecology · 2022Article
- Article
- Boosting the power of transcriptomics by developing an efficient gene expression profiling approach.Plant biotechnology journal · 2022Article
- Multi-dimensional leaf phenotypes reflect root system genotype in grafted grapevine over the growing season.GigaScience · 2021Article
- Application of the 3' mRNA-Seq using unique molecular identifiers in highly degraded RNA derived from formalin-fixed, paraffin-embedded tissue.BMC genomics · 2021Article
- Quantitative mapping of mRNA 3' ends in Pseudomonas aeruginosa reveals a pervasive role for premature 3' end formation in response to azithromycin.PLoS genetics · 2021Article
- Testing the Genomic Shock Hypothesis Using Transposable Element Expression in Yeast Hybrids.Frontiers in fungal biology · 2021Article
- Molecular basis of resistance to organophosphate insecticides in the New World screw-worm fly.Parasites & vectors · 2020Article
- Low RIN Value for RNA-Seq Library Construction from Long-Term Stored Seeds: A Case Study of Barley Seeds.Genes · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
One limitation of the widely used RNA-seq method is that long transcripts are represented by more reads than shorter transcripts, resulting in a biased estimation of expression levels. The 3' RNA-seq method, which yields only one sequence per transcript, bypasses this limitation. Here, RNA was extracted from two samples, in which we expected to find differentially expressed genes. Each was processed by both traditional and 3' RNA-seq protocols. Both methods yielded similar differentially expressed genes and estimated expression levels in a comparable way, confirming they both represent valid tools for RNA-seq analysis. Notably, however, we identified more differentially expressed transcripts with the 3' RNA-seq method, suggesting a greater power to detect expression variation using this method. Hence, when little genomic information is available for the species studied, the standard RNA-seq presents a better cost-benefit compromise, whereas for model species, the 3' RNA-seq method might more accurately detect differential expression.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.