ArticleBMC biotechnology2016
Expression of a fungal manganese peroxidase in Escherichia coli: a comparison between the soluble and refolded enzymes.
Article in BMC biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 30 citations in OpenAlex.
- Efficient extracellular expression and rapid screening of human-like recombinant gelatin in Komagataella phaffii.Scientific reports · 2025Article
- Bacillus subtilis spore surface display enhances manganese peroxidase stability and stress resistance.Bioresources and bioprocessing · 2025Article
- Degradation and detoxification of aflatoxin B1 by two peroxidase enzymes from Irpex lacteus F17.Bioprocess and biosystems engineering · 2025Article
- A mutant R70V/E166A of short manganese peroxidase showing MnApplied microbiology and biotechnology · 2023Article
- Article
- Textile Dye Biodecolorization by Manganese Peroxidase: A Review.Molecules (Basel, Switzerland) · 2021Review
- Simultaneous degradation of two mycotoxins enabled by a fusion enzyme in food-grade recombinant Kluyveromyces lactis.Bioresources and bioprocessing · 2021Article
- Assessing the Biodegradation of Vulcanised Rubber Particles by Fungi Using Genetic, Molecular and Surface Analysis.Frontiers in bioengineering and biotechnology · 2021Article
- Scalable High-Performance Production of Recombinant Horseradish Peroxidase fromInternational journal of molecular sciences · 2020Article
- Functional Expression and One-Step Protein Purification of Manganese Peroxidase 1 (rMnP1) fromInternational journal of molecular sciences · 2020Article
- Extracellular Oxidases of Basidiomycete Neonothopanus nambi: Isolation and Some Properties.Doklady. Biochemistry and biophysics · 2020Article
- Comprehensive investigation of a dye-decolorizing peroxidase and a manganese peroxidase from Irpex lacteus F17, a lignin-degrading basidiomycete.AMB Express · 2018Article
- Genomic and transcriptomic analyses reveal differential regulation of diverse terpenoid and polyketides secondary metabolites in Hericium erinaceus.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundManganese peroxidase (MnP) from Irpex lacteus F17 has been shown to have a strong ability to degrade recalcitrant aromatic pollutants. In this study, a recombinant MnP from I. lacteus F17 was expressed in Escherichia coli Rosetta (DE3) in the form of inclusion bodies, which were refolded to achieve an active enzyme. Further, we optimized the in vitro refolding conditions to increase the recovery yield of the recombinant protein production. Additionally, we attempted to express recombinant MnP in soluble form in E. coli, and compared its activity with that of refolded MnP.
resultsRefolded MnP was obtained by optimizing the in vitro refolding conditions, and soluble MnP was produced in the presence of four additives, TritonX-100, Tween-80, ethanol, and glycerol, through incubation at 16 °C. Hemin and Ca
conclusionsOur results showed that two forms of recombinant MnP could be expressed in E. coli by varying the culture conditions during protein expression.
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