ArticleNucleic acids research2017
Active 5' splice sites regulate the biogenesis efficiency of Arabidopsis microRNAs derived from intron-containing genes.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Small but big player: the important role of microRNAs in legume crops.Molecular genetics and genomics : MGG · 2026Review
- Rice and shine: a review of miRNA-mediated responses to abiotic stress inFrontiers in plant science · 2026Review
- A Brief Overview of the Epigenetic Regulatory Mechanisms in Plants.International journal of molecular sciences · 2025Review
- Review
- RNA helicase Brr2a promotes miRNA biogenesis by properly remodelling secondary structure of pri-miRNAs.Nature plants · 2024Article
- Biomolecular condensates in plant RNA silencing: insights into formation, function, and stress responses.The Plant cell · 2024Review
- MiRNAs differentially expressed in vegetative and reproductive organs ofRNA biology · 2024Article
- microRNA biogenesis and stabilization in plants.Fundamental research · 2023Review
- Hyponastic Leaves 1 Interacts with RNA Pol II to Ensure Proper Transcription of MicroRNA Genes.Plant & cell physiology · 2023Article
- Cotranscriptional RNA processing and modification in plants.The Plant cell · 2023Review
- Recent Insights into Plant miRNA Biogenesis: Multiple Layers of miRNA Level Regulation.Plants (Basel, Switzerland) · 2023Review
- microRNA production inFrontiers in plant science · 2023Review
- Chromatin-associated microprocessor assembly is regulated by the U1 snRNP auxiliary protein PRP40.The Plant cell · 2022Article
- Du13 encodes a CPlant biotechnology journal · 2022Article
- R-loops at microRNA encoding loci promote co-transcriptional processing of pri-miRNAs in plants.Nature plants · 2022Article
- PRP4KA phosphorylates SERRATE for degradation via 20Science advances · 2022Article
- Catalytic activities, molecular connections, and biological functions of plant RNA exosome complexes.The Plant cell · 2022Review
- MicroRNA biogenesis and activity in plant cell dedifferentiation stimulated by cell wall removal.BMC plant biology · 2022Article
- Recent advances in the regulation of plant miRNA biogenesis.RNA biology · 2021Review
- mRNA adenosine methylase (MTA) deposits mProceedings of the National Academy of Sciences of the United States of America · 2020Article
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Authors and funding
9 authors.
Funding
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Abstract
Arabidopsis, miR402 that is encoded within the first intron of a protein-coding gene At1g77230, is induced by heat stress. Its upregulation correlates with splicing inhibition and intronic proximal polyA site selection. It suggests that miR402 is not processed from an intron, but rather from a shorter transcript after selection of the proximal polyA site within this intron. Recently, introns and active 5' splice sites (5'ss') have been shown to stimulate the accumulation of miRNAs encoded within the first exons of intron-containing MIR genes. In contrast, we have observed the opposite effect of splicing inhibition on intronic miR402 production. Transient expression experiments performed in tobacco leaves revealed a significant accumulation of the intronic mature miR402 when the 5'ss of the miR402-hosting intron was inactivated. In contrast, when the miR402 stem-loop structure was moved into the first exon, mutation of the first-intron 5'ss resulted in a decrease in the miRNA level. Thus, the 5'ss controls the efficiency of miRNA biogenesis. We also show that the SERRATE protein (a key component of the plant microprocessor) colocalizes and interacts with several U1 snRNP auxiliary proteins. We postulate that SERRATE-spliceosome connections have a direct effect on miRNA maturation.
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Registered trials
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