ArticlePloS one2016
iTRAQ-Based Quantitative Proteomic Comparison of Early- and Late-Passage Human Dermal Papilla Cell Secretome in Relation to Inducing Hair Follicle Regeneration.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 32 citations in OpenAlex.
- Exosomal miRNA-218-5p derived from low-passage dermal papilla cells modulates hair follicle growth and development.Non-coding RNA research · 2026Article
- The human umbilical cord-mesenchymal stem cell secretome regulates hair growth and cycle transition by promoting methylthioadenosine synthesis via the PI3K/AKT/mTOR pathway.Stem cell research & therapy · 2025Article
- Aqueous Dispersion of Unmodified Fullerene C60: Stimulation of Hair Growth and Study of a New Molecular Target for Interaction.International journal of molecular sciences · 2025Article
- Therapeutic potential of stem cell-derived exosomes in hair regeneration: A systematic review.World journal of stem cells · 2025Article
- Microneedle-Based Approaches for Skin Disease Treatment.Nano-micro letters · 2025Review
- Various Three-Dimensional Culture Methods and Cell Types for Exosome Production.Tissue engineering and regenerative medicine · 2023Review
- Adipose-Derived Stem Cell Exosomes Antagonize the Inhibitory Effect of Dihydrotestosterone on Hair Follicle Growth by Activating Wnt/Stem cells international · 2023Article
- Defining ovine dermal papilla cell markers and identifying key signaling pathways regulating its intrinsic properties.Frontiers in veterinary science · 2023Article
- Effect ofBiology · 2022Article
- Stem cell secretome as a mechanism for restoring hair loss due to stress, particularly alopecia areata: narrative review.Journal of biomedical science · 2022Review
- iTRAQ-based proteome profiling revealed the role of Phytochrome A in regulating primary metabolism in tomato seedling.Scientific reports · 2021Article
- Mesenchymal Stem Cell Secretome for Dermatology Application: A Review.Clinical, cosmetic and investigational dermatology · 2021Review
- Dermal exosomes containing miR-218-5p promote hair regeneration by regulating β-catenin signaling.Science advances · 2020Article
- Glucose metabolism regulates expression of hair-inductive genes of dermal papilla spheres via histone acetylation.Scientific reports · 2020Article
- Sustained release of dermal papilla-derived extracellular vesicles from injectable microgel promotes hair growth.Theranostics · 2020Article
- miR-140-5p in Small Extracellular Vesicles From Human Papilla Cells Stimulates Hair Growth by Promoting Proliferation of Outer Root Sheath and Hair Matrix Cells.Frontiers in cell and developmental biology · 2020Article
- Alterations in transcriptome and antioxidant activity of naturally aged mice exposed to selenium-rich rice.Environmental science and pollution research international · 2019Article
- Elevated carbon dioxide levels lead to proteome-wide alterations for optimal growth of a fast-growing cyanobacterium, Synechococcus elongatus PCC 11801.Scientific reports · 2019Article
- Real-time iTRAQ-based proteome profiling revealed the central metabolism involved in nitrogen starvation induced lipid accumulation in microalgae.Scientific reports · 2017Article
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Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alopecia is an exceedingly prevalent problem that lacks effective therapy. Recently, research has focused on early-passage dermal papilla cells (DPCs), which have hair inducing activity both in vivo and in vitro. Our previous study indicated that factors secreted from early-passage DPCs contribute to hair follicle (HF) regeneration. To identify which factors are responsible for HF regeneration and why late-passage DPCs lose this potential, we collected 48-h-culture medium (CM) from both of passage 3 and 9 DPCs, and subcutaneously injected the DPC-CM into NU/NU mice. Passage 3 DPC-CM induced HF regeneration, based on the emergence of a white hair coat, but passage 9 DPC-CM did not. In order to identify the key factors responsible for hair induction, CM from passage 3 and 9 DPCs was analyzed by iTRAQ-based quantitative proteomic technology. We identified 1360 proteins, of which 213 proteins were differentially expressed between CM from early-passage vs. late-passage DPCs, including SDF1, MMP3, biglycan and LTBP1. Further analysis indicated that the differentially-expressed proteins regulated the Wnt, TGF-β and BMP signaling pathways, which directly and indirectly participate in HF morphogenesis and regeneration. Subsequently, we selected 19 proteins for further verification by multiple reaction monitoring (MRM) between the two types of CM. These results indicate DPC-secreted proteins play important roles in HF regeneration, with SDF1, MMP3, biglycan, and LTBP1 being potential key inductive factors secreted by dermal papilla cells in the regeneration of hair follicles.
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