ArticlePloS one2016
Reliability of Potential Pain Biomarkers in the Saliva of Healthy Subjects: Inter-Individual Differences and Intersession Variability.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 2 of them syntheses that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 2 syntheses or guidelines pooled it, 37 citations in OpenAlex.
- Pooled it
- Changes in salivary biomarkers of pain, anxiety, stress, and inflammation related to tooth movement during orthodontic treatment: a systematic review.Dental press journal of orthodontics · 2024Pooled it
- A Bayesian Modeling Approach to Optimize Longitudinal Biomarker Sampling Schedules Using Hormonal Data.American journal of human biology : the official journal of the Human Biology Council · 2026Article
- Determination of soluble tumor necrosis factor receptor II and secretory immunoglobulin A in saliva of patients with dementia.European archives of psychiatry and clinical neuroscience · 2024Article
- Potential use of salivary TNF-α as a vaccine-induced pain biomarker in people with cerebral palsy and communication disorders.PloS one · 2024Article
- Stress and sleep deprivation-related biomarkers in saliva in patients with retinitis pigmentosa.PloS one · 2024Observational
- Variability of salivary analytes under daily conditions and their implications for periodontitis biomarkers.Frontiers in dental medicine · 2024Article
- Article
- Exploring Non-Invasive Salivary Biomarkers for Acute Pain Diagnostics: A Comprehensive Review.Diagnostics (Basel, Switzerland) · 2023Review
- Assessment of salivary stress and pain biomarkers and their relation to self-reported pain intensity during orthodontic tooth movement: a longitudinal and prospective study.Journal of orofacial orthopedics = Fortschritte der Kieferorthopadie : Organ/official journal Deutsche Gesellschaft fur Kieferorthopadie · 2022Article
- Toward Composite Pain Biomarkers of Neuropathic Pain-Focus on Peripheral Neuropathic Pain.Frontiers in pain research (Lausanne, Switzerland) · 2022Review
- Correlation between Biomarkers of Pain in Saliva and PAINAD Scale in Elderly People with Cognitive Impairment and Inability to Communicate.Journal of clinical medicine · 2021Article
- Evaluation of Potential Pain Biomarkers in Saliva and Pain Perception After Corneal Advanced Surface Ablation Surgery.Clinical ophthalmology (Auckland, N.Z.) · 2020Article
- Article
- Testosterone modulation of ethanol effects on the µ-opioid receptor kinetics in castrated rats.Biomedical reports · 2019Article
- A Machine Learning Approach for the Identification of a Biomarker of Human Pain using fNIRS.Scientific reports · 2019Article
- Diffuse noxious inhibitory controls and brain networks are modulated in a testosterone-dependent manner in Sprague Dawley rats.Behavioural brain research · 2018Article
- Non-Invasive Objective Markers to Measure Pain: A Direction to Develop a Pain Device - A Narrative Review.Indian journal of palliative careReview
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimSalivary cortisol, α-amylase (sAA), secretory IgA (sIgA), testosterone, and soluble fraction of receptor II of TNFα (sTNFαRII) could serve as objective pain measures, but the normal variability of these potential biomarkers is unknown. PATIENTS &
methodsSaliva was collected with the passive secretion method from 34, pain-free subjects in two single samples at least 24 hours apart. Biomarker variation and intersession reliability were assessed with the intraclass correlation coefficient (ICC). Also, we calculated the within-subject standard deviation (Sw) and the reproducibility (2.77 × Sw) of intersession measures.
resultsSalivary cortisol, sAA, sIgA, testosterone, and sTNFαRII yielded the following ICCs: 0.53, 0.003, 0.88, 0.42 and 0.83, respectively. We found no statistically significant systematic differences between sessions in any biomarker except for testosterone, which showed a decrease on the second day (p<0.001). The reproducibility for salivary cortisol, sAA, sIgA, testosterone, and sTNFαRII were 0.46 ng/ml, 12.88 U/ml, 11.7 μg/ml, 14.54 pg/ml and 18.29 pg/ml, respectively. Cortisol, testosterone and TNFαRII measurement variability showed a positive correlation with the magnitude (p<0.002), but no relationship was found for sAA and sIgA.
conclusionsSalivary sIgA and sTNFαRII show a remarkable good reproducibility and, therefore, could be useful as pain biomarkers. When using the passive secretion method, intersession variations in salivary sIgA of more than 11.7 μg/ml may reflect true biomarker change. In the case of sTNFαRII this will depend of the magnitude. The estimates herein provided should help investigators and clinicians differentiate actual biomarker modification from measurement variability.
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