ArticlePloS one2016
Nicotine Induces Podocyte Apoptosis through Increasing Oxidative Stress.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed, 1 synthesis or guideline pooled it, 65 citations in OpenAlex.
- Pooled it
- Nicotine-Mediated Redox and Nitrosative Stress in Cardiorenal Health.Hypertension (Dallas, Tex. : 1979) · 2026Review
- Atmospheric stressors and kidney diseases.Nature reviews. Nephrology · 2026Review
- Association of liver enzyme abnormalities and tobacco exposure with unexplained recurrent spontaneous abortion risk: A case-control study.The Journal of international medical research · 2026Article
- Cigarette smoking and the development of persistent proteinuria in middle-aged Japanese men: the Kansai Healthcare Study.Clinical and experimental nephrology · 2026Article
- Nicotinism vs. Glomerulopathies-Smoking as a Risk Factor for Primary Glomerulopathies.Antioxidants (Basel, Switzerland) · 2025Review
- Production of Acetylcholine by Podocytes and its Protection from Kidney Injury in GN.Journal of the American Society of Nephrology : JASN · 2025Article
- Identification of Oxidative Stress-Associated Biomarkers in Chronic Obstructive Pulmonary Disease: An Integrated Bioinformatics Analysis.International journal of chronic obstructive pulmonary disease · 2025Article
- Protective effects of Apelin-13 on nicotine-induced H9c2 cardiomyocyte apoptosis and oxidative stress.Tobacco induced diseases · 2025Article
- Hydroxycotinine exhibits a stronger association with chronic kidney disease in smokers when compared to cotinine: Evidence from NHANES 2013-2018.Tobacco induced diseases · 2025Article
- Association Between Maternal Smoking, Isolated Proteinuria During Pregnancy and Preterm Birth: A Finnish Registry Analysis.Kidney international reports · 2025Article
- Exploring Vaping Patterns and Weight Management-Related Concerns among Adolescents and Young Adults: A Systematic Review.Journal of clinical medicine · 2024Review
- Dexpanthenol protects against nicotine-induced kidney injury by reducing oxidative stress and apoptosis through activation of the AKT/Nrf2/HO-1 pathway.Naunyn-Schmiedeberg's archives of pharmacology · 2024Article
- The hidden impact: the rate of nicotine metabolism and kidney health.Frontiers in endocrinology · 2024Article
- Nicotine exacerbates diabetic nephropathy through upregulation of Grem1 expression.Molecular medicine (Cambridge, Mass.) · 2023Article
- Classification, Perception, and Toxicity of Emerging Flavored Oral Nicotine Pouches.International journal of environmental research and public health · 2023Review
- Tobacco exposure in adults and children with proteinuric glomerulopathies: a NEPTUNE cohort study.BMC nephrology · 2023Observational
- Xenobiotics Delivered by Electronic Nicotine Delivery Systems: Potential Cellular and Molecular Mechanisms on the Pathogenesis of Chronic Kidney Disease.International journal of molecular sciences · 2022Review
- Article
- Effects of Smoking on Diabetic Nephropathy.Frontiers in clinical diabetes and healthcare · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundCigarette smoking plays an important role in the progression of chronic kidney disease (CKD). Nicotine, one of the major components of cigarette smoking, has been demonstrated to increase proliferation of renal mesangial cells. In this study, we examined the effect of nicotine on podocyte injury.
methodsTo determine the expression of nicotinic acetylcholine receptors (nAChR subunits) in podocytes, cDNAs and cell lysate of cultured human podocytes were used for the expression of nAChR mRNAs and proteins, respectively; and mouse renal cortical sections were subjected to immunofluorescant staining. We also studied the effect of nicotine on podocyte nephrin expression, reactive oxygen species (ROS) generation (via DCFDA loading followed by fluorometric analysis), proliferation, and apoptosis (morphologic assays). We evaluated the effect of nicotine on podocyte downstream signaling including phosphorylation of ERK1/2, JNK, and p38 and established causal relationships by using respective inhibitors. We used nAChR antagonists to confirm the role of nicotine on podocyte injury.
resultsHuman podocytes displayed robust mRNA and protein expression of nAChR in vitro studies. In vivo studies, mice renal cortical sections revealed co-localization of nAChRs along with synaptopodin. In vitro studies, nephrin expression in podocyte was decreased by nicotine. Nicotine stimulated podocyte ROS generation; nonetheless, antioxidants such as N-acetyl cysteine (NAC) and TEMPOL (superoxide dismutase mimetic agent) inhibited this effect of nicotine. Nicotine did not modulate proliferation but promoted apoptosis in podocytes. Nicotine enhanced podocyte phosphorylation of ERK1/2, JNK, and p38, and their specific inhibitors attenuated nicotine-induced apoptosis. nAChR antagonists significantly suppressed the effects of nicotine on podocyte.
conclusionsNicotine induces podocyte apoptosis through ROS generation and associated downstream MAPKs signaling. The present study provides insight into molecular mechanisms involved in smoking associated progression of chronic kidney disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.