ArticleCellular reprogramming2016
Effects of the Transforming Growth Factor Beta Signaling Pathway on the Differentiation of Chicken Embryonic Stem Cells into Male Germ Cells.
Article in Cellular reprogramming, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 25 citations in OpenAlex.
- Integrated metabolomic and transcriptomic analyses reveal that glutamine promotes chicken primordial germ cell‑like cell specification potentially through α-ketoglutarate.Animal bioscience · 2026Article
- Effects of dietary palygorskite supplementation on production performance and reproductive function of laying hens.Poultry science · 2026Article
- Analysis of the characteristics of microRNA expression profiles and reproductive regulation-mediated signalling pathways in testicular sertoli derived sEVs from Leizhou goats.Veterinary research communications · 2026Article
- Effect of HDAC9 on the differentiation of chicken embryonic stem cells into male germ cells.Animal reproduction · 2024Article
- Recent advances and future prospects in direct cardiac reprogramming.Nature cardiovascular research · 2023Review
- Dual regulatory actions of LncBMP4 on BMP4 promote chicken primordial germ cell formation.EMBO reports · 2022Article
- Article
- Study on the Function and Mechanism of Lin28B in the Formation of Chicken Primordial Germ Cells.Animals : an open access journal from MDPI · 2020Article
- C1EIP Functions as an Activator of ENO1 to Promote Chicken PGCs Formation via Inhibition of the Notch Signaling Pathway.Frontiers in genetics · 2020Article
- Efficient generation of male germ-like cells derived during co-culturing of adipose-derived mesenchymal stem cells with Sertoli cells under retinoic acid and testosterone induction.Stem cell research & therapy · 2019Article
- Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The objectives of the present study were to screen for key gene and signaling pathways involved in the production of male germ cells in poultry and to investigate the effects of the transforming growth factor beta (TGF-β) signaling pathway on the differentiation of chicken embryonic stem cells (ESCs) into male germ cells. The ESCs, primordial germ cells, and spermatogonial stem cells (SSCs) were sorted using flow cytometry for RNA sequencing (RNA-seq) technology. Male chicken ESCs were induced using 40 ng/mL of bone morphogenetic protein 4 (BMP4). The effects of the TGF-β signaling pathway on the production of chicken SSCs were confirmed by morphology, quantitative real-time polymerase chain reaction, and immunocytochemistry. One hundred seventy-three key genes relevant to development, differentiation, and metabolism and 20 signaling pathways involved in cell reproduction, differentiation, and signal transduction were identified by RNA-seq. The germ cells formed agglomerates and increased in number 14 days after induction by BMP4. During the induction process, the ESCs, Nanog, and Sox2 marker gene expression levels decreased, whereas expression of the germ cell-specific genes Stra8, Dazl, integrin-α6, and c-kit increased. The results indicated that the TGF-β signaling pathway participated in the differentiation of chicken ESCs into male germ cells.
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