ArticleScientific reports2016
Increased plasma VEGF levels following ischemic preconditioning are associated with downregulation of miRNA-762 and miR-3072-5p.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 2 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 2 syntheses or guidelines pooled it, 42 citations in OpenAlex.
- Remote ischemic conditioning for stroke: A critical systematic review.International journal of stroke : official journal of the International Stroke Society · 2024Pooled it
- Histological Findings After Aortic Cross-Clamping in Preclinical Animal Models.Journal of neuropathology and experimental neurology · 2021Pooled it
- Identification and validation of microglia-associated genes in ischemic stroke using single-cell and bulk RNA-seq.Molecular brain · 2025Article
- Investigating Molecular Mechanisms in Ischemic Preconditioning-Induced Resiliency to Severe Acute Global Cerebral Ischemia Using a Mouse Model of Chronic Cerebral Hypoperfusion.Cellular and molecular neurobiology · 2025Article
- miR-709 exerts an angiogenic effect through a FGF2 upregulation induced by a GSK3B downregulation.Scientific reports · 2024Article
- The roles of microRNAs in spinal cord ischemia-reperfusion injury.Neural regeneration research · 2022Review
- Non-pharmacological interventions for vascular health and the role of the endothelium.European journal of applied physiology · 2022Review
- Myocardial Protection and Current Cancer Therapy: Two Opposite Targets with Inevitable Cost.International journal of molecular sciences · 2022Review
- Remote ischemic preconditioning protects against cerebral ischemia injury in rats by upregulating miR-204-5p and activating the PINK1/Parkin signaling pathway.Metabolic brain disease · 2022Article
- Cardiac Exosomes in Ischemic Heart Disease- A Narrative Review.Diagnostics (Basel, Switzerland) · 2021Review
- MicroRNA-144 promotes remote limb ischemic preconditioning-mediated neuroprotection against ischemic stroke via PTEN/Akt pathway.Acta neurologica Belgica · 2021Article
- Non-Coding RNAs: Emerging Therapeutic Targets in Spinal Cord Ischemia-Reperfusion Injury.Frontiers in neurology · 2021Review
- Immune Modulation as a Key Mechanism for the Protective Effects of Remote Ischemic Conditioning After Stroke.Frontiers in neurology · 2021Review
- Effects of Remote Ischemic Conditioning on Hand Engagement in individuals with Spinal cord Injury (RICHES): protocol for a pilot crossover study.F1000Research · 2021Article
- Inositol-requiring enzyme-1 regulates phosphoinositide signaling lipids and macrophage growth.EMBO reports · 2020Article
- Time-Dependent miRNA Profile during Septic Acute Kidney Injury in Mice.International journal of molecular sciences · 2020Article
- MicroRNA files in the prevention of intestinal ischemia/reperfusion injury by hydrogen rich saline.Bioscience reports · 2020Article
- Ischemic Postconditioning Mitigates Retinopathy in Tree Shrews with Diabetic Cerebral Ischemia.Journal of diabetes research · 2020Article
- Unique molecular signatures of microRNAs in ocular fluids and plasma in diabetic retinopathy.PloS one · 2020Article
- Effects of Remote Ischemic Conditioning on Cerebral Hemodynamics in Ischemic Stroke.Neuropsychiatric disease and treatment · 2020Review
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ischemic preconditioning (IPC) has protective effects against ischemia-perfusion injury of organs. In the present study, we investigated the associated mechanisms after performing remote IPC (rIPC) of lower limbs by clamping abdominal aorta in mice. Subsequent experiments showed decreased damage and paralysis of lower limbs following spinal cord injury (SCI). Concomitantly, plasma vascular endothelial growth factor (VEGF) levels were increased 24 h after rIPC compared with those in sham-operated animals. In subsequent microRNA analyses, thirteen microRNAs were downregulated in exosomes 24 h after rIPC. Further studies of femoral CD34-positive bone marrow (BM) cells confirmed downregulation of these seven microRNAs 24 h after rIPC compared with those in sham-operated controls. Subsequent algorithm-based database searches suggested that two of the seven microRNAs bind to the 3' UTR of VEGF mRNA, and following transfection into CD34-positive BM cells, anti-miR-762, and anti-miR-3072-5p inhibitors led to increased VEGF concentrations. The present data suggest that rIPC transiently increases plasma VEGF levels by downregulating miR-762 and miR-3072-5p in CD34-positive BM cells, leading to protection against organ ischemia.
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