ArticleScientific reports2016
Efficient targeted mutagenesis of rice and tobacco genomes using Cpf1 from Francisella novicida.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 133 papers.
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Who cites it
133 citing papers in PubMed, 306 citations in OpenAlex.
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- CRISPR-Mediated Genome Editing in Peanuts: Unlocking Trait Improvement for a Sustainable Future.Plants (Basel, Switzerland) · 2025Review
- CRISPR/Cas-Mediated Optimization of Soybean Shoot Architecture for Enhanced Yield.International journal of molecular sciences · 2025Review
- Advances in Functional Genomics for Exploring Abiotic Stress Tolerance Mechanisms in Cereals.Plants (Basel, Switzerland) · 2025Review
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- CRISPR-Cas applications in agriculture and plant research.Nature reviews. Molecular cell biology · 2025Review
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- Genome editing for grass improvement and future agriculture.Horticulture research · 2025Article
- PAM-relaxed and temperature-tolerant CRISPR-Mb3Cas12a single transcript unit systems for efficient singular and multiplexed genome editing in rice, maize, and tomato.Plant biotechnology journal · 2025Article
- CRISPR/Cas technology: fueling the future of Biofuel production with sugarcane.Functional & integrative genomics · 2024Review
- Context effects on repair of 5'-overhang DNA double-strand breaks induced by Cas12a in Arabidopsis.Plant direct · 2024Article
- Rice Yellow Mottle Virus resistance by genome editing of the Oryza sativa L. ssp. japonica nucleoporin gene OsCPR5.1 but not OsCPR5.2.Plant biotechnology journal · 2024Article
- Cas12a and MAD7, genome editing tools for breeding.Breeding science · 2024Article
- Harnessing noncanonical crRNAs to improve functionality of Cas12a orthologs.Cell reports · 2024Article
- Recent Advances in Tomato Gene Editing.International journal of molecular sciences · 2024Review
- High-efficiency genome editing by Cas12a ribonucleoprotein complex in Euglena gracilis.Microbial biotechnology · 2024Article
73 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CRISPR/Cas9 systems are nowadays applied extensively to effect genome editing in various organisms including plants. CRISPR from Prevotella and Francisella 1 (Cpf1) is a newly characterized RNA-guided endonuclease that has two distinct features as compared to Cas9. First, Cpf1 utilizes a thymidine-rich protospacer adjacent motif (PAM) while Cas9 prefers a guanidine-rich PAM. Cpf1 could be used as a sequence-specific nuclease to target AT-rich regions of a genome that Cas9 had difficulty accessing. Second, Cpf1 generates DNA ends with a 5' overhang, whereas Cas9 creates blunt DNA ends after cleavage. "Sticky" DNA ends should increase the efficiency of insertion of a desired DNA fragment into the Cpf1-cleaved site using complementary DNA ends. Therefore, Cpf1 could be a potent tool for precise genome engineering. To evaluate whether Cpf1 can be applied to plant genome editing, we selected Cpf1 from Francisella novicida (FnCpf1), which recognizes a shorter PAM (TTN) within known Cpf1 proteins, and applied it to targeted mutagenesis in tobacco and rice. Our results show that targeted mutagenesis had occurred in transgenic plants expressing FnCpf1 with crRNA. Deletions of the targeted region were the most frequently observed mutations. Our results demonstrate that FnCpf1 can be applied successfully to genome engineering in plants.
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