ArticleScience translational medicine2016
Imaging mitochondrial dynamics in human skin reveals depth-dependent hypoxia and malignant potential for diagnosis.
Article in Science translational medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed.
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- Decoding elastin-collagen resemblance in keloid scar through label-free imaging and machine learning.Journal of biomedical optics · 2026Article
- Intraoperative biopsy imaging of lung cancer risk.Communications medicine · 2026Article
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- Advanced Microscopy Techniques.Recent results in cancer research. Fortschritte der Krebsforschung. Progres dans les recherches sur le cancer · 2026Review
- Label-free human skin imaging with enhanced molecular contrast via time-resolved fluorescence and advanced phasor analysis.Communications biology · 2025Article
- Multi-modal label-free imaging of cellular metabolism and oxidative stress in 3D brain tissue models.Communications biology · 2025Article
- Unravelling the genetics and epigenetics of the ageing tumour microenvironment in cancer.Nature reviews. Cancer · 2025Review
- Combining In Vivo 2-Photon Imaging with Photoactivatable Fluorescent Labeling Shows Low Rates of Mitochondrial Dynamics in Skeletal Muscle.Medicine and science in sports and exercise · 2025Article
- Fast and label-free 3D virtual H&E histology via active phase modulation-assisted dynamic full-field OCT.Npj imaging · 2025Article
- Motion-tolerant 3D volumetric multimodality microscopy imaging of human skin with subcellular resolution and extended field-of-view.Communications biology · 2025Article
- Synergistic label-free fluorescence imaging and miRNA studies reveal dynamic human neuron-glial metabolic interactions following injury.Science advances · 2024Article
- Rapid, high-resolution, non-destructive assessments of metabolic and morphological homogeneity uniquely identify high-grade cervical precancerous lesions.bioRxiv : the preprint server for biology · 2024Article
- Two photon imaging probe with highly efficient autofluorescence collection at high scattering and deep imaging conditions.Biomedical optics express · 2024Article
- Noninvasive Imaging Techniques for Monitoring Cellular Response to Treatment in Stable Vitiligo.The Journal of investigative dermatology · 2024Article
- A New Approach to Vascular Screening: Identification of Impaired Vascular Function Using the FMSF Technique.Sensors (Basel, Switzerland) · 2024Review
- A 20 MHz Repetition Rate, Sub-Picosecond Ti-Sapphire Laser for Fiber Delivery in Nonlinear Microscopy of the Skin.Life (Basel, Switzerland) · 2024Article
- Formalin fixation and paraffin embedding interfere with the preservation of optical metabolic assessments based on endogenous NAD(P)H and FAD two-photon excited fluorescence.Biomedical optics express · 2023Article
- Label-Free Optical Metabolic Imaging in Cells and Tissues.Annual review of biomedical engineering · 2023Review
- Review
1 more citing papers are in PubMed but not listed here.
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10 authors.
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Abstract
Active changes in mitochondrial structure and organization facilitate cellular homeostasis. Because aberrant mitochondrial dynamics are implicated in a variety of human diseases, their assessment is potentially useful for diagnosis, therapy, and disease monitoring. Because current techniques for evaluating mitochondrial morphology are invasive or necessitate mitochondria-specific dyes, their clinical translation is limited. We report that mitochondrial dynamics can be monitored in vivo, within intact human skin by relying entirely on endogenous two-photon-excited fluorescence from the reduced metabolic coenzyme nicotinamide adenine dinucleotide (NADH). We established the sensitivity of this approach with in vivo, fast temporal studies of arterial occlusion-reperfusion, which revealed acute changes in the mitochondrial metabolism and dynamics of the lower human epidermal layers. In vitro hypoxic-reperfusion studies validated that the in vivo outcomes were a result of NADH fluorescence changes. To demonstrate the diagnostic potential of this approach, we evaluated healthy and cancerous human skin epithelia. Healthy tissues displayed consistent, depth-dependent morphological and mitochondrial organization patterns that varied with histological stratification and intraepithelial mitochondrial protein expression. In contrast, these consistent patterns were absent in cancerous skin lesions. We exploited these differences to successfully differentiate healthy from cancerous tissues using a predictive classification approach. Collectively, these results demonstrate that our label-free, automated, near real-time assessments of mitochondrial organization-relying solely on endogenous contrast-could be useful for accurate, noninvasive in vivo diagnosis.
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