ReviewEssays in biochemistry2016
Properties of alternative microbial hosts used in synthetic biology: towards the design of a modular chassis.
Review in Essays in biochemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- Advances in the sustainable biosynthesis of valuable terpenoid flavor compounds and precursors in micro-organisms.Biotechnology for biofuels and bioproducts · 2025Review
- Fine-Tuning Genetic Circuits via Host Context and RBS Modulation.ACS synthetic biology · 2025Article
- Influence of Environmental Conditions on the Escape Rates of Biocontained Genetically Engineered Microbes.Environmental science & technology · 2024Article
- Synthetic biology toolkit of Ralstonia eutropha (Cupriavidus necator).Applied microbiology and biotechnology · 2024Review
- Review
- DetSpace: a web server for engineering detectable pathways for bio-based chemical production.Nucleic acids research · 2024Article
- Bacterial microcompartments as a next-generation metabolic engineering tool: utilizing nature's solution for confining challenging catabolic pathways.Biochemical Society transactions · 2024Review
- Multiplex CRISPR-Cas Genome Editing: Next-Generation Microbial Strain Engineering.Journal of agricultural and food chemistry · 2024Review
- Machine learning analysis of RB-TnSeq fitness data predicts functional gene modules inmSystems · 2024Article
- Recent advances in producing food additive L-malate: Chassis, substrate, pathway, fermentation regulation and application.Microbial biotechnology · 2023Review
- Recent advances in genetic tools for engineering probiotic lactic acid bacteria.Bioscience reports · 2023Article
- A Framework for the Systematic Selection of Biosensor Chassis for Environmental Synthetic Biology.ACS synthetic biology · 2022Review
- Picking the right metaphors for addressing microbial systems: economic theory helps understanding biological complexity.International microbiology : the official journal of the Spanish Society for Microbiology · 2021Article
- Contextual dependencies expand the re-usability of genetic inverters.Nature communications · 2021Article
- Xylose-Inducible Promoter Tools forApplied and environmental microbiology · 2020Article
- Synthetic control of plasmid replication enables target- and self-curing of vectors and expedites genome engineering ofMetabolic engineering communications · 2020Article
- Harnessing evolutionary diversification of primary metabolism for plant synthetic biology.The Journal of biological chemistry · 2019Review
- Engineering and modification of microbial chassis for systems and synthetic biology.Synthetic and systems biotechnology · 2019Review
- Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
The chassis is the cellular host used as a recipient of engineered biological systems in synthetic biology. They are required to propagate the genetic information and to express the genes encoded in it. Despite being an essential element for the appropriate function of genetic circuits, the chassis is rarely considered in their design phase. Consequently, the circuits are transferred to model organisms commonly used in the laboratory, such as Escherichia coli, that may be suboptimal for a required function. In this review, we discuss some of the properties desirable in a versatile chassis and summarize some examples of alternative hosts for synthetic biology amenable for engineering. These properties include a suitable life style, a robust cell wall, good knowledge of its regulatory network as well as of the interplay of the host components with the exogenous circuits, and the possibility of developing whole-cell models and tuneable metabolic fluxes that could allow a better distribution of cellular resources (metabolites, ATP, nucleotides, amino acids, transcriptional and translational machinery). We highlight Pseudomonas putida, widely used in many different biotechnological applications as a prominent organism for synthetic biology due to its metabolic diversity, robustness and ease of manipulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.