ArticleBMC evolutionary biology2016
Revealing hidden species diversity in closely related species using nuclear SNPs, SSRs and DNA sequences - a case study in the tree genus Milicia.
Article in BMC evolutionary biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 90 citations in OpenAlex.
- Article
- Low Genetic Diversity and Complex Population Structure in Black Piranha (Ecology and evolution · 2025Article
- Genetic characterization of a group of commercial African timber species: From genomics to barcoding.PloS one · 2023Article
- Genomics Reveal Admixture and Unexpected Patterns of Diversity in a Parapatric Pair of Butterflies.Genes · 2021Article
- Timber identification of Autranella, Baillonella and Tieghemella in the taxonomically challenging Sapotaceae family.Plant methods · 2021Article
- Candidate Genes for the High-Altitude Adaptations of Two Mountain Pine Taxa.International journal of molecular sciences · 2021Article
- Article
- Unraveling the Phylogenomic Relationships of the Most Diverse African Palm GenusPlants (Basel, Switzerland) · 2020Article
- Miocene Diversification in the Savannahs Precedes Tetraploid Rainforest Radiation in the African Tree GenusFrontiers in plant science · 2020Article
- Chloroplast and mitochondrial genetic variation of larches at the Siberian tundra-taiga ecotone revealed by de novo assembly.PloS one · 2019Article
- Isolation of microsatellite loci in the African tree species Staudtia kamerunensis (Myristicaceae) using high-throughput sequencing.Molecular biology reports · 2018Article
- Genome-wide single-nucleotide polymorphism data reveal cryptic species within cryptic freshwater snail species-The case of theEcology and evolution · 2018Article
- Evaluation of multiple approaches to identify genome-wide polymorphisms in closely related genotypes of sweet cherry (Computational and structural biotechnology journal · 2017Article
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Authors and funding
11 authors at 6 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSpecies delimitation in closely related plant taxa can be challenging because (i) reproductive barriers are not always congruent with morphological differentiation, (ii) use of plastid sequences might lead to misinterpretation, (iii) rare species might not be sampled. We revisited molecular-based species delimitation in the African genus Milicia, currently divided into M. regia (West Africa) and M. excelsa (from West to East Africa). We used 435 samples collected in West, Central and East Africa. We genotyped SNP and SSR loci to identify genetic clusters, and sequenced two plastid regions (psbA-trnH, trnC-ycf6) and a nuclear gene (At103) to confirm species' divergence and compare species delimitation methods. We also examined whether ecological niche differentiation was congruent with sampled genetic structure.
resultsWest African M. regia, West African and East African M. excelsa samples constituted three well distinct genetic clusters according to SNPs and SSRs. In Central Africa, two genetic clusters were consistently inferred by both types of markers, while a few scattered samples, sympatric with the preceding clusters but exhibiting leaf traits of M. regia, were grouped with the West African M. regia cluster based on SNPs or formed a distinct cluster based on SSRs. SSR results were confirmed by sequence data from the nuclear region At103 which revealed three distinct 'Fields For Recombination' corresponding to (i) West African M. regia, (ii) Central African samples with leaf traits of M. regia, and (iii) all M. excelsa samples. None of the plastid sequences provide indication of distinct clades of the three species-like units. Niche modelling techniques yielded a significant correlation between niche overlap and genetic distance.
conclusionsOur genetic data suggest that three species of Milicia could be recognized. It is surprising that the occurrence of two species in Central Africa was not reported for this well-known timber tree. Globally, our work highlights the importance of collecting samples in a systematic way and the need for combining different nuclear markers when dealing with species complexes. Recognizing cryptic species is particularly crucial for economically exploited species because some hidden taxa might actually be endangered as they are merged with more abundant species.
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