ArticleeLife2016
Differentiation alters stem cell nuclear architecture, mechanics, and mechano-sensitivity.
Article in eLife, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 105 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
105 citing papers in PubMed, 196 citations in OpenAlex.
- Nuclear mechanobiology: a brief history and five unresolved questions.Nucleus (Austin, Tex.) · 2026Article
- The Mechano-Genomic Frontier: Orchestrating Nuclear Deformation for Craniomaxillofacial Bone Regeneration.Journal of clinical medicine · 2026Review
- Multiscale Architecture and Mechanics of the Cell Nucleus: Implications for Disease, Bioengineering and Nanomedicine.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Expansion Microscopy reveals changes in cellular and extracellular structures between healthy and perturbed tendons.bioRxiv : the preprint server for biology · 2026Article
- Chromatin architecture and physical constriction cooperate in phenotype switching and cancer cell dissemination.bioRxiv : the preprint server for biology · 2026Article
- Stress fiber traction force reshapes chromatin accessibility and YAP binding to direct diverse transcriptional programs in mesenchymal stem cells.Mechanobiology in medicine · 2026Article
- Mechanical memory in cells: mechanisms, effects, and applications in regenerative medicine.Frontiers in bioengineering and biotechnology · 2026Review
- Genetically reprogrammed BMSCs with Lamin B1 depletion enhance bone regeneration for osteoporosis therapy.Frontiers in bioengineering and biotechnology · 2026Article
- Chromatin remodeling and epigenetic regulation of mesenchymal stem/stromal cells in osteoarthritis.Frontiers in genetics · 2026Review
- Mechanostimulation-Induced Cell Adhesion and Interaction with the Extracellular Matrix.Biomolecules · 2025Review
- Mechanosensitive genomic enhancers potentiate the cellular response to matrix stiffness.Science (New York, N.Y.) · 2025Article
- Heterogeneity as a feature: unraveling chromatin's role in nuclear mechanics.Nucleus (Austin, Tex.) · 2025Review
- Rapid restoration of cell phenotype and matrix forming capacity following transient nuclear softening.Acta biomaterialia · 2025Article
- Lamin A/C protects chromatin accessibility during mechanical loading in human skeletal muscle.Cell communication and signaling : CCS · 2025Article
- In vivo mechano-tissue engineering by hydrogels capable of transmitting intercellular mechanical stress.Nature communications · 2025Article
- Nuclear pores safeguard the integrity of the nuclear envelope.Nature cell biology · 2025Article
- Depletion of SUN1/2 induces heterochromatin accrual in mesenchymal stem cells during adipogenesis.Communications biology · 2025Article
- Nanoscale distribution of bioactive ligands on biomaterials regulates cell mechanosensing through translocation of actin into the nucleus.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Mechanotransductive N-cadherin binding induces differentiation in human neural stem cells.Mechanobiology in medicine · 2025Article
- Bone-derived nanoparticles (BNPs) enhance osteogenic differentiationNanoscale advances · 2025Article
45 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
Mesenchymal stem cell (MSC) differentiation is mediated by soluble and physical cues. In this study, we investigated differentiation-induced transformations in MSC cellular and nuclear biophysical properties and queried their role in mechanosensation. Our data show that nuclei in differentiated bovine and human MSCs stiffen and become resistant to deformation. This attenuated nuclear deformation was governed by restructuring of Lamin A/C and increased heterochromatin content. This change in nuclear stiffness sensitized MSCs to mechanical-loading-induced calcium signaling and differentiated marker expression. This sensitization was reversed when the 'stiff' differentiated nucleus was softened and was enhanced when the 'soft' undifferentiated nucleus was stiffened through pharmacologic treatment. Interestingly, dynamic loading of undifferentiated MSCs, in the absence of soluble differentiation factors, stiffened and condensed the nucleus, and increased mechanosensitivity more rapidly than soluble factors. These data suggest that the nucleus acts as a mechanostat to modulate cellular mechanosensation during differentiation.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.