ArticleScientific reports2016
Asian Citrus Psyllid RNAi Pathway - RNAi evidence.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 80 citations in OpenAlex.
- Root uptake, translocation and persistence of EAB-specific dsRNA in ash seedlings.Scientific reports · 2025Article
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- Characteristics of environmental RNAi in potato psyllid,Frontiers in physiology · 2022Article
- RNAi inFrontiers in plant science · 2022Article
- Double-Strand RNA (dsRNA) Delivery Methods in Insects: Diaphorina citri.Methods in molecular biology (Clifton, N.J.) · 2022Article
- RNAi Feeding Bioassay: A Protocol for dsRNA Screening Against Asian Citrus Psyllid and Related Hemipteran Insects.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Transcriptome of the Maize Leafhopper (Microbiology spectrum · 2021Article
- Innate and adaptive resistance to RNAi: a major challenge and hurdle to the development of double stranded RNA-based pesticides.3 Biotech · 2021Review
- Silencing of Aquaporin Homologue Accumulates Uric Acid and Decreases the Lifespan of the Asian Citrus Psyllid,Insects · 2021Article
- Optimizing Efficient RNAi-Mediated Control of Hemipteran Pests (Psyllids, Leafhoppers, Whitefly): Modified Pyrimidines in dsRNA Triggers.Plants (Basel, Switzerland) · 2021Article
- Crowdsourced Identification of Potential Target Genes for CTV Induced Gene Silencing for Controlling the Citrus Greening VectorFrontiers in physiology · 2021Article
- Genome-enabled insights into the biology of thrips as crop pests.BMC biology · 2020Article
- Article
- An Interaction Network of RNA-Binding Proteins Involved inMolecular & cellular proteomics : MCP · 2020Article
- RNAi-Based Functional Genomics in Hemiptera.Insects · 2020Review
- Article
- Gene silencing of Diaphorina citri candidate effectors promotes changes in feeding behaviors.Scientific reports · 2020Article
- First transcriptome of the Neotropical pest Euschistus heros (Hemiptera: Pentatomidae) with dissection of its siRNA machinery.Scientific reports · 2020Article
- Insecticidal Gene Silencing by RNAi in the Neotropical Region.Neotropical entomology · 2020Review
- Citrus Genetic Engineering for Disease Resistance: Past, Present and Future.International journal of molecular sciences · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Diaphorina citri, known as the Asian citrus psyllid, is an important pest of citrus because it transmits a phloem-limited bacteria strongly implicated in huanglongbing (citrus greening disease). Emerging biotechnologies, such as RNA interference, could provide a new sustainable and environmentally friendly strategy for the management of this pest. In this study, genome and functional analysis were performed to verify whether the RNAi core genes are present in the Asian psyllid genome and if the RNAi machinery could be exploited to develop a management strategy for this pest. Analyses of RNAi-related genes in the Asian citrus psyllid genome showed an absence of sequences encoding R2D2, a dsRNA-binding protein that functions as a cofactor of Dicer-2 in Drosophila. Nevertheless, bioassays using an in Planta System showed that the Asian citrus psyllid was very sensitive to ingested dsRNA, demonstrating a strong RNAi response. A small dose of dsRNA administered through a citrus flush was enough to trigger the RNAi mechanism, causing significant suppression of the targeted transcript, and increased psyllid mortality. This study provides evidence of a functional RNAi machinery, which could be further exploited to develop RNAi based management strategies for the control of the Asian citrus psyllid.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.