ArticleScientific reports2016
A-Kinase Anchoring Protein 4 (AKAP4) is an ERK1/2 substrate and a switch molecule between cAMP/PKA and PKC/ERK1/2 in human spermatozoa.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 53 citations in OpenAlex.
- Sperm Membrane: Molecular Implications and Strategies for Cryopreservation in Productive Species.Animals : an open access journal from MDPI · 2025Review
- Melatonin regulates rabbit sperm motility and kinematics via the MT1/PKC signaling pathway.Animal bioscience · 2025Article
- A-Kinase Anchor Protein 4 (proAKAP4): Protein Molecule-Based Fertility Marker of Indonesian Dairy Bull and Its Correlation With Frozen-Thawed Sperm Quality.Veterinary medicine international · 2025Article
- Spatial Organization of the Sperm Cell Glycoproteome.Molecular & cellular proteomics : MCP · 2025Article
- Assessing the Relationship between proAKAP4 Level and Longevity of Sexed Sperm Quality after Thawing.Veterinary sciences · 2024Article
- Identification of hub genes and their expression profiling for predicting buffalo (Bubalus bubalis) semen quality and fertility.Scientific reports · 2023Article
- ADAD1 is required for normal translation of nuclear pore and transport protein transcripts in spermatids of Mus musculus†.Biology of reproduction · 2023Article
- Seminal plasma protein profiles and testosterone levels as biomarker semen quality of candidate Madura bulls.Journal of advanced veterinary and animal research · 2023Article
- ProAKAP4 Concentration Is Related to Sperm Motility and Motile Sperm Subpopulations in Frozen-Thawed Horse Semen.Animals : an open access journal from MDPI · 2022Article
- Comparative analysis of the testes from wild-type and Alkbh5-knockout mice using single-cell RNA sequencing.G3 (Bethesda, Md.) · 2022Article
- Characterization of the Testis-Specific Angiotensin Converting Enzyme (tACE)-Interactome during Bovine Sperm Capacitation.Current issues in molecular biology · 2022Article
- CD73-Adenosine AFrontiers in pharmacology · 2022Article
- Identification of CD8Frontiers in immunology · 2022Article
- Quantitative phosphoproteomics analyses reveal the regulatory mechanisms related to frozen-thawed sperm capacitation and acrosome reaction in yak (Frontiers in physiology · 2022Article
- The regulatory role of PDE4B in the progression of inflammatory function study.Frontiers in pharmacology · 2022Review
- Comparing the Effect of Different Antibiotics in Frozen-Thawed Ram Sperm: Is It Possible to Avoid Their Addition?Frontiers in veterinary science · 2021Article
- Comparative Proteomics and Phosphoproteomics Analysis Reveal the Possible Breed Difference in Yorkshire and Duroc Boar Spermatozoa.Frontiers in cell and developmental biology · 2021Article
- Altered Gene Expression in the Testis of Infertile Patients with Nonobstructive Azoospermia.Computational and mathematical methods in medicine · 2021Article
- Phosphoproteomics and Bioinformatics Analyses Reveal Key Roles of GSK-3 and AKAP4 in Mouse Sperm Capacitation.International journal of molecular sciences · 2020Article
- ProAKAP4 as Novel Molecular Marker of Sperm Quality in Ram: An Integrative Study in Fresh, Cooled and Cryopreserved Sperm.Biomolecules · 2020Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mammalian spermatozoa undergo capacitation and acrosome reaction in order to fertilize the egg. The PKC-ERK1/2 pathway plays an important role in human spermatozoa motility, capacitation and the acrosome reaction. Here we demonstrate that ERK1/2 phosphorylates proAKAP4 on Thr265 in human spermatozoa in vitro and in vivo. Cyclic AMP (cAMP) had no effect on ERK1/2 activity in human spermatozoa, but stimulated the MAPK in mouse pituitary LβT2 gonadotrope cells. cAMP via PKA attenuates PKC-dependent ERK1/2 activation only in the presence of proAKAP4. St-HT31, which disrupts PKA-regulatory subunit II (PKA-RII) binding to AKAP abrogates the inhibitory effect of cAMP in human spermatozoa and in HEK293T cells expressing proAKAP4. In transfected HEK293T cells, PMA relocated proAKAP4, but not proAKAP4-T265A to the Golgi in an ERK1/2-dependnet manner. Similarly, AKAP4 is localized to the spermatozoa principal piece and is relocated to the mid-piece and the postacrosomal region by PMA. Furthermore, using capacitated sperm we found that cAMP reduced PMA-induced ERK1/2 activation and acrosome reaction. Thus, the physiological role of the negative crosstalk between the cAMP/PKA/AKAP4 and the PKC/ERK1/2 pathways is to regulate capacitation and acrosome reaction.
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