ArticleNucleic acids research2017
MRPrimerV: a database of PCR primers for RNA virus detection.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 24 citations in OpenAlex.
- Microbial communities in filth flies collected from dairy and poultry farms for supplemental animal feed.Journal of economic entomology · 2026Article
- RAZOR: a database of PCR primers targeting human respiratory viruses.NAR genomics and bioinformatics · 2026Article
- In-silico matching of SARS-CoV-2 genes and oligonucleotides using the Simple Oligo Matching Tool.Genes & genomics · 2025Article
- BioInnovate AI: A Machine Learning Platform for Rapid PCR Assay Design in Emerging Infectious Disease Diagnostics.Diagnostics (Basel, Switzerland) · 2025Article
- MiPRIME: an integrated and intelligent platform for mining primer and probe sequences of microbial species.Bioinformatics (Oxford, England) · 2024Article
- Diagnostics of Ebola virus.Frontiers in public health · 2023Review
- Identification of conserved regions from 230,163 SARS-CoV-2 genomes and their use in diagnostic PCR primer design.Genes & genomics · 2022Article
- Orchestrating an Optimized Next-Generation Sequencing-Based Cloud Workflow for Robust Viral Identification during Pandemics.Biology · 2021Article
- GPrimer: a fast GPU-based pipeline for primer design for qPCR experiments.BMC bioinformatics · 2021Article
- Rapid metagenomics analysis of EMS vehicles for monitoring pathogen load using nanopore DNA sequencing.PloS one · 2019Article
- A multiplex one-tube nested real time RT-PCR assay for simultaneous detection of respiratory syncytial virus, human rhinovirus and human metapneumovirus.Virology journal · 2018Article
- Zika Virus Liquid Biopsy: A Dendritic Ru(bpy)ACS central science · 2018Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many infectious diseases are caused by viral infections, and in particular by RNA viruses such as MERS, Ebola and Zika. To understand viral disease, detection and identification of these viruses are essential. Although PCR is widely used for rapid virus identification due to its low cost and high sensitivity and specificity, very few online database resources have compiled PCR primers for RNA viruses. To effectively detect viruses, the MRPrimerV database (http://MRPrimerV.com) contains 152 380 247 PCR primer pairs for detection of 1818 viruses, covering 7144 coding sequences (CDSs), representing 100% of the RNA viruses in the most up-to-date NCBI RefSeq database. Due to rigorous similarity testing against all human and viral sequences, every primer in MRPrimerV is highly target-specific. Because MRPrimerV ranks CDSs by the penalty scores of their best primer, users need only use the first primer pair for a single-phase PCR or the first two primer pairs for two-phase PCR. Moreover, MRPrimerV provides the list of genome neighbors that can be detected using each primer pair, covering 22 192 variants of 532 RefSeq RNA viruses. We believe that the public availability of MRPrimerV will facilitate viral metagenomics studies aimed at evaluating the variability of viruses, as well as other scientific tasks.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.