ArticleNucleic acids research2017
Engineering robust and tunable spatial structures with synthetic gene circuits.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 52 citations in OpenAlex.
- Gaseous liquid nitrogen-assisted cryo-printing strategies for challenging volumetric anisotropic tissues fabrication.Science advances · 2026Article
- Engineering oncolytic bacteria as precision cancer therapeutics: design principles, therapeutic strategies, and translational perspectives.Protein & cell · 2026Review
- Sonogenetics-controlled synthetic designer cells for cancer therapy in tumor mouse models.Cell reports. Medicine · 2024Article
- Protein-based bandpass filters for controlling cellular signaling with chemical inputs.Nature chemical biology · 2024Article
- Modularized Design and Construction of Tunable Microbial Consortia with Flexible Topologies.ACS synthetic biology · 2024Article
- Article
- Synthetic spatial patterning in bacteria: advances based on novel diffusible signals.Microbial biotechnology · 2022Review
- Frequency dependent growth of bacteria in living materials.Frontiers in bioengineering and biotechnology · 2022Article
- Emergent spatiotemporal population dynamics with cell-length control of synthetic microbial consortia.PLoS computational biology · 2021Article
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- Engineering AraC to make it responsive to light instead of arabinose.Nature chemical biology · 2021Article
- Biotechnological Innovations and Therapeutic Application of Pediococcus and Lactic Acid Bacteria: The Next-Generation Microorganism.Frontiers in bioengineering and biotechnology · 2021Review
- Interpretation of morphogen gradients by a synthetic bistable circuit.Nature communications · 2020Article
- Genetically engineered control of phenotypic structure in microbial colonies.Nature microbiology · 2020Article
- From Microbial Communities to Distributed Computing Systems.Frontiers in bioengineering and biotechnology · 2020Review
- Spatiotemporal Dynamics of Synthetic Microbial Consortia in Microfluidic Devices.ACS synthetic biology · 2019Article
- Synthetic Mutualism and the Intervention Dilemma.Life (Basel, Switzerland) · 2019Review
- Rational engineering of synthetic microbial systems: from single cells to consortia.Current opinion in microbiology · 2018Review
- Synthetic biology in probiotic lactic acid bacteria: At the frontier of living therapeutics.Current opinion in biotechnology · 2018Review
- Contribution of YthA, a PspC Family Transcriptional Regulator of Lactococcus lactis F44 Acid Tolerance and Nisin Yield: a Transcriptomic Approach.Applied and environmental microbiology · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Controllable spatial patterning is a major goal for the engineering of biological systems. Recently, synthetic gene circuits have become promising tools to achieve the goal; however, they need to possess both functional robustness and tunability in order to facilitate future applications. Here we show that, by harnessing the dual signaling and antibiotic features of nisin, simple synthetic circuits can direct Lactococcus lactis populations to form programmed spatial band-pass structures that do not require fine-tuning and are robust against environmental and cellular context perturbations. Although robust, the patterns are highly tunable, with their band widths specified by the external nisin gradient and cellular nisin immunity. Additionally, the circuits can direct cells to consistently generate designed patterns, even when the gradient is driven by structured nisin-producing bacteria and the patterning cells are composed of multiple species. A mathematical model successfully reproduces all of the observed patterns. Furthermore, the circuits allow us to establish predictable structures of synthetic communities and controllable arrays of cellular stripes and spots in space. This study offers new synthetic biology tools to program spatial structures. It also demonstrates that a deep mining of natural functionalities of living systems is a valuable route to build circuit robustness and tunability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.