ArticlemBio2016
Gene Function Analysis in the Ubiquitous Human Commensal and Pathogen Malassezia Genus.
Article in mBio, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 54 citations in OpenAlex.
- Host skin lipids triggerbioRxiv : the preprint server for biology · 2026Article
- Commensal yeast Malassezia produces tryptophan metabolites to promote tissue homeostasis via the aryl hydrocarbon receptor in mice.Nature microbiology · 2026Article
- Skin mycobiota-mediated antagonism against Staphylococcus aureus through a modified fatty acid.Current biology : CB · 2025Article
- Uncovering Microbial Diversity and Community Structure of Black Spots Residing in Tomb Mural Painting.Microorganisms · 2025Article
- Malassezia responds to environmental pH signals through the conserved Rim/Pal pathway.bioRxiv : the preprint server for biology · 2024Article
- What are the 100 most cited fungal genera?Studies in mycology · 2024Article
- High-quality genome assembly and genetic transformation system ofMicrobiology spectrum · 2024Article
- Frequent transitions in mating-type locus chromosomal organization inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Frequent transitions in mating-type locus chromosomal organization inbioRxiv : the preprint server for biology · 2023Article
- The human pathobiontProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Many ways, one microorganism: Several approaches to study Malassezia in interactions with model hosts.PLoS pathogens · 2022Review
- Article
- Skin Commensal FungusJournal of microbiology and biotechnology · 2021Review
- Azole resistance mechanisms in pathogenicAntimicrobial agents and chemotherapy · 2021Article
- Molecular Tools for the Yeast Papiliotrema terrestris LS28 and Identification of Yap1 as a Transcription Factor Involved in Biocontrol Activity.Applied and environmental microbiology · 2021Article
- A case of anti- pityriasis versicolor therapy that preserves healthy mycobiome.BMC dermatology · 2020Article
- HGT in the human and skin commensalProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Article
- 5-fluorocytosine resistance is associated with hypermutation and alterations in capsule biosynthesis in Cryptococcus.Nature communications · 2020Article
- Review
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 3 countries.
Funding
Abstract
The genus Malassezia includes 14 species that are found on the skin of humans and animals and are associated with a number of diseases. Recent genome sequencing projects have defined the gene content of all 14 species; however, to date, genetic manipulation has not been possible for any species within this genus. Here, we develop and then optimize molecular tools for the transformation of Malassezia furfur and Malassezia sympodialis using Agrobacterium tumefaciens delivery of transfer DNA (T-DNA) molecules. These T-DNAs can insert randomly into the genome. In the case of M. furfur, targeted gene replacements were also achieved via homologous recombination, enabling deletion of the ADE2 gene for purine biosynthesis and of the LAC2 gene predicted to be involved in melanin biosynthesis. Hence, the introduction of exogenous DNA and direct gene manipulation are feasible in Malassezia species. IMPORTANCE: Species in the genus Malassezia are a defining component of the microbiome of the surface of mammals. They are also associated with a wide range of skin disease symptoms. Many species are difficult to culture in vitro, and although genome sequences are available for the species in this genus, it has not been possible to assess gene function to date. In this study, we pursued a series of possible transformation methods and identified one that allows the introduction of DNA into two species of Malassezia, including the ability to make targeted integrations into the genome such that genes can be deleted. This research opens a new direction in terms of now being able to analyze gene functions in this little understood genus. These tools will contribute to define the mechanisms that lead to the commensalism and pathogenicity in this group of obligate fungi that are predominant on the skin of mammals.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.