ArticleThe Journal of biological chemistry2017
Real Time, Spatial, and Temporal Mapping of the Distribution of c-di-GMP during Biofilm Development.
Article in The Journal of biological chemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.
- Microbial Community Associations With Listeria monocytogenes in Food Processing Environments: A Systematic Review and Meta-Analysis.Comprehensive reviews in food science and food safety · 2025Pooled it
- Participation of the Transcriptional Regulator Fur in Modulating Biofilm Formation of the Salmonid PathogenInternational journal of molecular sciences · 2026Article
- Surface materials and biofilm formation affect tropodithietic acid biosynthesis gene expression inSustainable microbiology · 2026Article
- Understanding the development of bacterial colony: Physiology, new technology, and modeling.Quantitative biology (Beijing, China) · 2025Review
- eATP sensing by the purinergic receptor PA2072 for allosteric modulation in intracellular c-di-GMP signaling.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Dispersal Mechanisms in Biofilm Control: Characteristics, Induction, Impacts, and Therapeutic Potential.Current microbiology · 2025Review
- Updates on therapeutic targeting of diguanylate cyclase for addressing bacterial infections: A comprehensive review.World journal of microbiology & biotechnology · 2025Review
- Article
- A Label-Free Approach for Relative Spatial Quantitation of c-di-GMP in Microbial Biofilms.Analytical chemistry · 2024Article
- DNA at the center of mammalian innate immune recognition of bacterial biofilms.Trends in immunology · 2024Review
- A label-free approach for relative spatial quantitation of c-di-GMP in microbial biofilms.bioRxiv : the preprint server for biology · 2023Article
- The Pseudomonas aeruginosa Biofilm Matrix Protein CdrA Has Similarities to Other Fibrillar Adhesin Proteins.Journal of bacteriology · 2023Review
- The biofilm life cycle: expanding the conceptual model of biofilm formation.Nature reviews. Microbiology · 2022Review
- Elevated c-di-GMP Levels and Expression of the Type III Secretion System Promote Corneal Infection by Pseudomonas aeruginosa.Infection and immunity · 2022Article
- Controlling Biofilm Development Through Cyclic di-GMP Signaling.Advances in experimental medicine and biology · 2022Review
- Phenotypic and integrated analysis of a comprehensiveFrontiers in microbiology · 2022Article
- Carbon starvation of Pseudomonas aeruginosa biofilms selects for dispersal insensitive mutants.BMC microbiology · 2021Article
- Anti-biofilm Approach in Infective Endocarditis Exposes New Treatment Strategies for Improved Outcome.Frontiers in cell and developmental biology · 2021Review
- Accumulation of dead cells from contact killing facilitates coexistence in bacterial biofilms.Journal of the Royal Society, Interface · 2020Article
- Biofilm dispersion.Nature reviews. Microbiology · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP) is a dynamic intracellular signaling molecule that plays a central role in the biofilm life cycle. Current methodologies for the quantification of c-di-GMP are typically based on chemical extraction, representing end point measurements. Chemical methodologies also fail to take into consideration the physiological heterogeneity of the biofilm and thus represent an average c-di-GMP concentration across the entire biofilm. To address these problems, a ratiometric, image-based quantification method has been developed based on expression of the green fluorescence protein (GFP) under the control of the c-di-GMP-responsive cdrA promoter (Rybtke, M. T., Borlee, B. R., Murakami, K., Irie, Y., Hentzer, M., Nielsen, T. E., Givskov, M., Parsek, M. R., and Tolker-Nielsen, T. (2012) Appl. Environ. Microbiol. 78, 5060-5069). The methodology uses the cyan fluorescent protein (CFP) as a biomass indicator and the GFP as a c-di-GMP reporter. Thus, the CFP/GFP ratio gives the effective c-di-GMP per biomass. A binary mask was applied to alleviate background fluorescence, and fluorescence was calibrated against known c-di-GMP concentrations. Using flow cells for biofilm formation, c-di-GMP showed a non-uniform distribution across the biofilm, with concentrated hot spots of c-di-GMP. Additionally, c-di-GMP was found to be localized at the outer boundary of mature colonies in contrast to a uniform distribution in early stage, small colonies. These data demonstrate the application of a method for the in situ, real time quantification of c-di-GMP and show that the amount of this biofilm-regulating second messenger was dynamic with time and colony size, reflecting the extent of biofilm heterogeneity in real time.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.