ArticleBMC complementary and alternative medicine2016
The anti-Staphylococcus aureus activity of the phenanthrene fraction from fibrous roots of Bletilla striata.
Article in BMC complementary and alternative medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed.
- Article
- Study on Process Optimization and Antioxidant Activity of Polysaccharide fromMolecules (Basel, Switzerland) · 2023Article
- Meroterpenoids and Steroids from the Marine-Derived FungusMolecules (Basel, Switzerland) · 2022Article
- Article
- Discovery and identification of potential anti-melanogenic active constituents of Bletilla striata by zebrafish model and molecular docking.BMC complementary medicine and therapies · 2022Article
- Cytotoxic Indole-Diterpenoids from the Marine-Derived FungusMarine drugs · 2021Article
- The combined antibacterial effects of sodium new houttuyfonate and berberine chloride against growing and persistent methicillin-resistant and vancomycin-intermediate Staphylococcus aureus.BMC microbiology · 2020Article
- De novo sequencing of Bletilla striata (Orchidaceae) transcriptome and identification of genes involved in polysaccharide biosynthesis.Genetics and molecular biology · 2020Article
- Effective fraction of Bletilla striata reduces the inflammatory cytokine production induced by water and organic extracts of airborne fine particulate matter (PMBMC complementary and alternative medicine · 2019Article
- Characterization of the anti-Staphylococcus aureus fraction from Penthorum chinense Pursh stems.BMC complementary and alternative medicine · 2019Article
- Chemical Constituents, Pharmacologic Properties, and Clinical Applications ofFrontiers in pharmacology · 2019Review
- Chemical Constituents of the Marine-Derived FungusMarine drugs · 2018Article
- Phenanthrene Antibiotic Targets Bacterial Membranes and KillsFrontiers in microbiology · 2018Article
- Article
- Review
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7 authors.
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Abstract
backgroundBletillae Rhizoma, the tuber of Bletilla striata, has been used in Chinese traditional medicine to treat infectious diseases. Chemical studies indicated that phenanthrene was one of the most important components of the herb, with a broad spectrum of antibiotic activity against Gram-positive bacteria. The objective of this study was to further characterize the antibacterial activity of the phenanthrene fraction from the fibrous root of the pseudobulb of B. striata.
methodsThe phenanthrene fraction (EF60) from the ethanol extract of fibrous roots of Bletilla striata pseudobulbs was isolated using polyamide column chromatography. The antibacterial activity of the fraction was evaluated in vitro using a 96-well microtiter plate and microbroth dilution method. The cytotoxicity of EF60 against mammalian cells was tested by hemolysis and MTT assays.
resultsEF60 was obtained using alcohol extraction and polyamide column chromatography, with a yield of 14.9 g per 1 kg of the fibrous roots of B. striata. In vitro tests indicated that EF60 was active against all tested strains of Staphylococcus aureus, including clinical isolates and methicillin-resistant S. aureus (MRSA). The minimum inhibitory concentration (MIC) values of EF60 against these pathogens ranged from 8 to 64 μg/mL. Minimum bactericidal concentration tests demonstrated that EF60 was bactericidal against S. aureus 3304 and ATCC 29213 and was bacteriostatic against S. aureus 3211, ATCC 25923, and ATCC 43300. Consistently, the time-kill assay indicated that EF60 could completely kill S. aureus ATCC 29213 at 2× the MIC within 3 h but could kill less than two logarithmic units of ATCC 43300, even at 4× the MIC within 24 h. The postantibiotic effects (PAE) of EF60 (4× MIC) against strains 29213 and 43300 were 2.0 and 0.38 h, respectively. Further studies indicated that EF60 (160 μg/mL) showed no cytotoxicity against human erythrocytes, and was minimally toxic to Human Umbilical Vein Endothelial Cells with an IC
conclusionsOur studies indicated that EF60 is worthy of further investigation as a potential phytotherapeutic agent for treating infections caused by S. aureus and MRSA.
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