ArticleScientific reports2016
ECM proteins in a microporous scaffold influence hepatocyte morphology, function, and gene expression.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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Who cites it
25 citing papers in PubMed, 42 citations in OpenAlex.
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- In Vitro Human Liver Model for Toxicity Assessment with Clinical and Preclinical Instrumentation.Pharmaceutics · 2024Article
- Galactosylation of rat natural scaffold for MSC differentiation into hepatocyte-like cells: A comparative analysis of 2D vs. 3D cell culture techniques.Biochemistry and biophysics reports · 2023Article
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- Optimization of extracellular matrix for primary human hepatocyte cultures using mixed collagen-Matrigel matrices.EXCLI journal · 2023Article
- Fabrication of Conductive Tissue Engineering Nanocomposite Films Based on Chitosan and Surfactant-Stabilized Graphene Dispersions.Polymers · 2022Article
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- Review on Multicomponent Hydrogel Bioinks Based on Natural Biomaterials for Bioprinting 3D Liver Tissues.Frontiers in bioengineering and biotechnology · 2022Review
- Generation of Hepatobiliary Cell Lineages from Human Induced Pluripotent Stem Cells: Applications in Disease Modeling and Drug Screening.International journal of molecular sciences · 2021Review
- Biomaterial-based cell delivery strategies to promote liver regeneration.Biomaterials research · 2021Review
- The Microfluidic Environment Reveals a Hidden Role of Self-Organizing Extracellular Matrix in Hepatic Commitment and Organoid Formation of hiPSCs.Cell reports · 2020Article
- Gaining New Biological and Therapeutic Applications into the Liver with 3D In Vitro Liver Models.Tissue engineering and regenerative medicine · 2020Review
- Engineering liver microtissues for disease modeling and regenerative medicine.Advanced functional materials · 2020Article
- Functionalisation of a heat-derived and bio-inert albumin hydrogel with extracellular matrix by air plasma treatment.Scientific reports · 2020Article
- Development of Scaffolds with Adjusted Stiffness for Mimicking Disease-Related Alterations of Liver Rigidity.Journal of functional biomaterials · 2020Article
- Thermoresponsive Inverted Colloidal Crystal Hydrogel Scaffolds for Lymphoid Tissue Engineering.Advanced healthcare materials · 2020Article
- Current Perspective: 3D Spheroid Models Utilizing Human-Based Cells for Investigating Metabolism-Dependent Drug-Induced Liver Injury.Frontiers in medical technology · 2020Review
- 3D culture of functional human iPSC-derived hepatocytes using a core-shell microfiber.PloS one · 2020Article
- Intact extracellular matrix component promotes maintenance of liver-specific functions and larger aggregates formation of primary rat hepatocytes.Regenerative therapy · 2019Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is well known that a three-dimensional (3D) culture environment and the presence of extracellular matrix (ECM) proteins facilitate hepatocyte viability and maintenance of the liver-specific phenotype in vitro. However, it is not clear whether specific ECM components such as collagen or fibronectin differentially regulate such processes, especially in 3D scaffolds. In this study, a series of ECM-functionalized inverted colloidal crystal (ICC) microporous scaffolds were fabricated and their influence on Huh-7.5 cell proliferation, morphology, hepatic-specific functions, and patterns of gene expression were compared. Both collagen and fibronectin promoted albumin production and liver-specific gene expression of Huh-7.5 cells, compared with the bare ICC scaffold. Interestingly, cells in the fibronectin-functionalized scaffold exhibited different aggregation patterns to those in the collagen-functionalized scaffold, a variation that could be related to the distinct mRNA expression levels of cell adhesion-related genes. Based on these results, we can conclude that different ECM proteins, such as fibronectin and collagen, indeed play distinct roles in the phenotypic regulation of cells cultured in a 3D environment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.