The activation of the IFNβ induction/signaling pathway in porcine alveolar macrophages by porcine reproductive and respiratory syndrome virus is variable.

Christopher C Overend, Junru Cui, Marvin J Grubman, Antonio E Garmendia

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Article in Veterinary research communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it

0cells of the map it votes in

6citing papers in PubMed

2.3field-weighted citation impact, top 12% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 10 citations in OpenAlex.

A novel strategy for optimal component formula of anti-PRRSV from natural compounds using tandem mass tag labeled proteomic analyses.BMC veterinary research · 2022
Article
Differential responses in placenta and fetal thymus at 12 days post infection elucidate mechanisms of viral level and fetal compromise following PRRSV2 infection.BMC genomics · 2020
Article
In vitro immune responses of porcine alveolar macrophages reflect host immune responses against porcine reproductive and respiratory syndrome viruses.BMC veterinary research · 2018
Article
Transcriptional profiles of PBMCs from pigs infected with three genetically diverse porcine reproductive and respiratory syndrome virus strains.Molecular biology reports · 2018
Article
Nsp1α of Porcine Reproductive and Respiratory Syndrome Virus Strain BB0907 Impairs the Function of Monocyte-Derived Dendritic Cells via the Release of Soluble CD83.Journal of virology · 2018
Article
The Nucleocapsid Protein and Nonstructural Protein 10 of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Enhance CD83 Production via NF-κB and Sp1 Signaling Pathways.Journal of virology · 2017
Article

4 · The record

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5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Christopher C OverendDepartment of Pathobiology and Veterinary Science, University of Connecticut, 61 North Eagleville Rd, Storrs, CT, 06269, USA.

Junru CuiDepartment of Pathobiology and Veterinary Science, University of Connecticut, 61 North Eagleville Rd, Storrs, CT, 06269, USA.

Marvin J GrubmanPlum Island Animal Disease Center,USDA/ARS, Greenport, NY, USA.

Antonio E GarmendiaDepartment of Pathobiology and Veterinary Science, University of Connecticut, 61 North Eagleville Rd, Storrs, CT, 06269, USA. Antonio.Garmendia@uconn.edu.

University of Connecticut · USPlum Island Animal Disease Center · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundIt has been recognized that the expression of type I interferon (IFNα/β) may be suppressed during infection with porcine reproductive, respiratory syndrome virus (PRRSV). This causes profound negative effects on both the innate and adaptive immunity of the host resulting in persistence of infection.

objectiveTest the effects of PRRSV infection of porcine alveolar macrophages (PAMs), the main target cell, on the expression of interferon beta (IFNβ) and downstream signaling events.

methodsIn order to examine those effects, PAMs harvested from lungs of healthy PRRSV-free animals were infected with virulent, attenuated, infectious clone-derived chimeric viruses, or field PRRS virus strains. Culture supernatants from the infected PAMs were tested for IFNβ protein expression by means of indirect ELISA and for bioactivity by a vesicular stomatitis virus plaque reduction assay. The expression of the Mx protein was assayed to ascertain signaling events.

resultsThese experiments demonstrated that PRRSV does induce variably, the expression of bioactive IFNβ protein in the natural host cell. To further elucidate the effects of PRRSV infection on IFNβ signaling, Mx-1 an interferon stimulated gene (ISG), was also tested for expression. Interestingly, Mx-1 expression by infected PAMs generally correlated with IFNβ production.

conclusionThe results of this study demonstrate that the induction of IFNβ and signaling in PAMs after PRRSV infection is variable.

Indexed as

Gene Expression RegulationHost-Pathogen InteractionsSignal TransductionAnimalsEnzyme-Linked Immunosorbent AssayInterferon-betaLungMacrophages, AlveolarMyxovirus Resistance ProteinsPorcine Reproductive and Respiratory SyndromePorcine respiratory and reproductive syndrome virusSwineInterferon-betaMyxovirus Resistance ProteinsInterferon betaMx-1 innate immunityPorcine reproductive respiratory syndrome virus

Identifiers

PMID27896670

DOI10.1007/s11259-016-9665-6

OpenAlexW2559388670

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.