Evidence map›Paper›PMID 27896542›Full record

ArticleInflammation2017

Immunomodulatory Effects of Adjuvants CPG, MPLA, and BCG on the Derp2-Induced Acute Asthma at Early Life in an Animal Model of BALB/c Mice.

V Mohammadi-Shahrokhi, A Rezaei, A Andalib, A Rahnama, A Jafarzadeh, N Eskandari

Abstract read
PubMed Publisher
In one paragraph

Article in Inflammation, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.8field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Review
  3. Vaccine Potential of Mycobacterial Antigens against Asthma.Medical principles and practice : international journal of the Kuwait University, Health Science Centre · 2020
    Review
  4. Review
  5. Article
  6. Dietary intake is associated with respiratory health outcomes and DNA methylation in children with asthma.Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology · 2017
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

V Mohammadi-ShahrokhiDepartment of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
A RezaeiDepartment of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
A AndalibDepartment of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
A RahnamaDepartment of Pathology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
A JafarzadehDepartment of Immunology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran. Jafarzadeh14@yahoo.com.
N EskandariDepartment of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. neskandari@med.mui.ac.ir.
Isfahan University of Medical Sciences · IRKerman University of Medical Sciences · IRRafsanjan University of Medical Sciences · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The Th1- and Treg cell-related immune responses play key roles in the modulation of Th2 cell-related allergic disorders. The aim was to evaluate the effects of CPG, MPLA, and BCG on the number of Th1-, Th2-, and Treg cell-related parameters in an animal model of asthma. BALB/c mice were divided into five groups and immunized subcutaneously (SC) on days 1, 15, and 22 with allergen Derp2. Three groups of mice were pretreated SC on days 0, 14, and 21 with CPG, CPG + MPLA, or CPG + BCG. All mice were then challenged intranasally with Derp2 on days 28-37. Blood samples were collected from the retro-orbital sinus, on days 0, 23, and 40. The serum levels of IL-4, IFN-γ, IgE, and IgG2a were measured using ELISA technique. The blood number of Th1 and Treg cells was determined using flow cytometry. At the sensitization phase, the number of Th1 and the serum levels of IFN-γ and IgG2a were significantly increased in the Derp2-sensitized group pretreated with CPG plus MPLA, and the number of Treg cells was significantly elevated in Derp2-sensitized mice pretreated with CPG or CPG plus MPLA as compared with that in Derp2-sensitized control mice. At the challenge phase, the number of Th1 was significantly elevated in Derp2-sensitized mice pretreated with CPG plus MPLA, CPG plus BCG, or CPG; the count of Treg cells was significantly increased in Derp2-sensitized mice pretreated with CPG plus BCG group; and the levels of IFN-γ and IgG2a were significantly enhanced in the Derp2-sensitized group pretreated with CPG plus MPLA in comparison with those in Derp2-sensitized control mice. The scores of inflammation and mucus secretion in the lung were significantly decreased in the Derp2-sensitized group pretreated with CPG, BCG, and CPG plus MPLA in comparison with those in the Derp2-sensitized control group. These results showed that BCG, MPLA, and CPG modulate Th1-, Th2-, and Treg-related parameters and ameliorate lung inflammatory parameters in a mouse model of asthma.

Indexed as

Acute DiseaseAdjuvants, ImmunologicAnimalsAntigens, DermatophagoidesAsthmaImmunomodulationInflammationLipid AMiceMice, Inbred BALB CMycobacterium bovisOligodeoxyribonucleotidesTh1 CellsTh2 CellsT-Lymphocytes, RegulatoryAdjuvants, ImmunologicAntigens, DermatophagoidesCPG-oligonucleotideDerp2 allergen, Dermatophagoides pteronyssinusLipid Amonophosphoryl lipid AOligodeoxyribonucleotidesasthmaBCGCPG-ODNinflammationmiceMPLA

Identifiers

PMID27896542
OpenAlexW2558264230

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.