ArticleBiomedical optics express2016
Simple approach to three-color two-photon microscopy by a fiber-optic wavelength convertor.
Article in Biomedical optics express, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Tailored Multi-Color Dispersive Wave Formation in Quasi-Phase-Matched Exposed Core Fibers.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- A rapid denoised contrast enhancement method digitally mimicking an adaptive illumination in submicron-resolution neuronal imaging.iScience · 2022Article
- Single-laser-based simultaneous four-wavelength excitation source for femtosecond two-photon fluorescence microscopy.Biomedical optics express · 2021Article
- Low noise, self-phase-modulation-enabled femtosecond fiber sources tunable in 740-1236 nm for wide two-photon fluorescence microscopy applications.Biomedical optics express · 2021Article
- Serial imaging of micro-agents and cancer cell spheroids in a microfluidic channel using multicolor fluorescence microscopy.PloS one · 2021Article
- Ultrafast Fiber Lasers: An Expanding Versatile Toolbox.iScience · 2020Review
- Frontiers in Intravital Multiphoton Microscopy of Cancer.Cancer reports (Hoboken, N.J.) · 2020Review
- SyncRGB-FLIM: synchronous fluorescence imaging of red, green and blue dyes enabled by ultra-broadband few-cycle laser excitation and fluorescence lifetime detection.Biomedical optics express · 2019Article
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Authors and funding
4 authors.
Funding
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Abstract
A simple approach to multi-color two-photon microscopy of the red, green, and blue fluorescent indicators was reported based on an ultra-compact 1.03-μm femtosecond laser and a nonlinear fiber. Inside the nonlinear fiber, the 1.03-μm laser pulses were simultaneously blue-shifted to 0.6~0.8 μm and red-shifted to 1.2~1.4 μm region by the Cherenkov radiation and fiber Raman gain effects. The wavelength-shifted 0.6~0.8 μm and 1.2~1.4 μm radiations were co-propagated with the residual non-converted 1.03-μm pulses inside the same nonlinear fiber to form a fiber-output three-color femtosecond source. The application of the multi-wavelength sources on multi-color two-photon fluorescence microscopy were also demonstrated. Overall, due to simple system configuration, convenient wavelength conversion, easy wavelength tunability within the entire 0.7~1.35 μm bio-penetration window and less requirement for high power and bulky light sources, the simple approach to multi-color two-photon microscopy could be widely applicable as an easily implemented and excellent research tool for future biomedical and possibly even clinical applications.
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