ArticleBiomedical optics express2016
Intracellular dynamics measurements with full field optical coherence tomography suggest hindering effect of actomyosin contractility on organelle transport.
Article in Biomedical optics express, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 40 citations in OpenAlex.
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- Sensitivity Enhancement of Dynamic Full-Field Optical Coherence Tomography Using Ratio-Free Detection and Partial-Field Illumination for Retinal Organoid Imaging.Bioengineering (Basel, Switzerland) · 2026Article
- Using Principal Components Analysis to Visualize Motion and Mitigate Artifacts in Dynamic Optical Coherence Tomography.Journal of biophotonics · 2026Article
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- Dynamic contrast in dual-beam optical coherence tomography imaging of human skin.Biomedical optics express · 2025Article
- Review of dynamic optical coherence tomography for intracellular motility: signals, metrics, and their applications [Invited].Biomedical optics express · 2025Review
- Guide to dynamic OCT data analysis.Biomedical optics express · 2025Article
- Line-field dynamic optical coherence tomography platform for volumetric assessment of biological tissues.Biomedical optics express · 2024Article
- Label-free visualization and quantification of the drug-type-dependent response of tumor spheroids by dynamic optical coherence tomography.Scientific reports · 2024Article
- Comparative oncology chemosensitivity assay for personalized medicine using low-coherence digital holography of dynamic light scattering from cancer biopsies.Scientific reports · 2024Article
- Evaluating the feasibility and predictive accuracy of biodynamic imaging to platinum-based chemotherapy response in esophageal adenocarcinoma.Frontiers in oncology · 2024Article
- Evaluating the performance of OCT in assessing static and potential dynamic properties of the retinal ganglion cells and nerve fiber bundles in the living mouse eye.Biomedical optics express · 2023Article
- Dynamic full-field optical coherence tomography module adapted to commercial microscopes allows longitudinal in vitro cell culture study.Communications biology · 2023Article
- Label-free drug response evaluation of human derived tumor spheroids using three-dimensional dynamic optical coherence tomography.Scientific reports · 2023Article
- Dynamic optical coherence tomography for cell analysis [Invited].Biomedical optics express · 2023Review
- Optical coherence microscopy with a split-spectrum image reconstruction method for temporal-dynamics contrast-based imaging of intracellular motility.Biomedical optics express · 2023Article
- Label free optical transmission tomography for biosystems: intracellular structures and dynamics.Biomedical optics express · 2022Article
- Present Application and Perspectives of Organoid Imaging Technology.Bioengineering (Basel, Switzerland) · 2022Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intracellular motion can be quantitatively monitored in tissues using coherence-gated microscopic techniques. With full-field optical coherence tomography (FFOCT), the use of high numerical aperture microscope objectives provides a high resolution mapping of intracellular dynamics that are probed with subwavelength sensitivity. In the upper temporal bandwidth that we have used (1-6 Hz) the main contribution to the dynamic signal arises from the overall dynamical, optically heterogeneous cytoplasm. We propose a method to specifically study the impact of actomyosin contractility on the intracellular dynamic signal by performing high throughput, comparative measurements of multicellular aggregates with and without blebbistatin action, a selective inhibitor of class-II myosins that disrupts actomyosin contractile activity. Our results indicate a significant increase in the fraction of the signal that decorrelates within 1 second after inhibition of contractility. This observation mitigates the anticipated importance of actomyosin contractile forces to directly move organelles, but highlights their role in hindering organelle transport via their stiffening effect of the viscoelastic cytoplasm.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.