ArticleJournal of animal science and biotechnology2016
Proteomic identification of cryostress in epididymal spermatozoa.
Article in Journal of animal science and biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 38 citations in OpenAlex.
- Recent advances in cryotolerance biomarkers for semen preservation in frozen form-A systematic review.PloS one · 2024Pooled it
- Proteomic dynamics of bull sperm during post-testicular maturation.BMC genomics · 2026Article
- Proteomic signatures of sperm and extracellular vesicles associated with sperm freezability in Holstein bulls.Scientific reports · 2026Article
- Extender development for optimal cryopreservation of buck sperm to increase reproductive efficiency of goats.Frontiers in veterinary science · 2025Review
- Identification of Biomarkers Affecting Cryopreservation Recovery Ratio in Ram Spermatozoa Using Tandem Mass Tags (TMT)-Based Quantitative Proteomics Approach.Animals : an open access journal from MDPI · 2023Article
- In Vitro versus Cryo-Induced Capacitation of Bovine Spermatozoa, Part 2: Changes in the Expression Patterns of Selected Transmembrane Channels and Protein Kinase A.International journal of molecular sciences · 2022Article
- Expression ofInternational journal of molecular sciences · 2022Article
- Age-Dependent Variations in Functional Quality and Proteomic Characteristics of Canine (International journal of molecular sciences · 2022Article
- Proteomic Analysis of Intracellular and Membrane-Associated Fractions of Canine (Animals : an open access journal from MDPI · 2022Article
- Cryoprotectants-Free Vitrification and Conventional Freezing of Human Spermatozoa: A Comparative Transcript Profiling.International journal of molecular sciences · 2022Article
- The cryopreservation process induces alterations in proteins associated with bull sperm quality: The equilibration process could be a probable critical control point.Frontiers in endocrinology · 2022Article
- Unraveling Subcellular and Ultrastructural Changes During Vitrification of Human Spermatozoa: Effect of a Mitochondria-Targeted Antioxidant and a Permeable Cryoprotectant.Frontiers in cell and developmental biology · 2021Article
- Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality.International journal of molecular sciences · 2020Review
- Effect of Cooling and Freezing on Llama (Frontiers in veterinary science · 2020Article
- Freezability biomarkers in bull epididymal spermatozoa.Scientific reports · 2019Article
- Cryopreservation Differentially Alters the Proteome of Epididymal and Ejaculated Pig Spermatozoa.International journal of molecular sciences · 2019Article
- Human leukemia cells (HL-60) proteomic and biological signatures underpinning cryo-damage are differentially modulated by novel cryo-additives.GigaScience · 2019Article
- Influence of cryopreservation on theToxicology reports · 2019Article
- Expression pattern of prohibitin, capping actin protein of muscle Z-line beta subunit and tektin-2 gene in Murrah buffalo sperm and its relationship with sperm motility.Asian-Australasian journal of animal sciences · 2018Article
- Serine Protease Inhibitor SERPINE2 Reversibly Modulates Murine Sperm Capacitation.International journal of molecular sciences · 2018Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCryopreservation of epididymal spermatozoa is important in cases in which it is not possible to collect semen using normal methods, as the sudden death of an animal or a catastrophic injury. However, the freezing and thawing processes cause stress to spermatozoa, including cold shock, osmotic damage, and ice crystal formation, thereby reducing sperm quality. We assessed the motility (%), motion kinematics, capacitation status, and viability of spermatozoa using computer-assisted sperm analysis and Hoechst 33258/chlortetracycline fluorescence staining. Moreover, we identified proteins associated with cryostress using a proteomic approach and performed western blotting to validate two-dimensional electrophoresis (2-DE) results using two commercial antibodies.
resultsCryopreservation reduced viability (%), motility (%), straight-line velocity (VSL), average path velocity (VAP), amplitude of lateral head displacement (ALH), and capacitated spermatozoa, whereas straightness (STR) and the acrosome reaction increased after cryopreservation (
conclusionThe results of the current study provide information on epididymal sperm proteome dynamics and possible protein markers of cryo-stress during cryopreservation. This information will further the basic understanding of cryopreservation and aid future studies aiming to identify the mechanism of cryostress responses.
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Registered trials
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