ArticleCell & bioscience2016
Downregulation of long non-coding RNA H19 promotes P19CL6 cells proliferation and inhibits apoptosis during late-stage cardiac differentiation via miR-19b-modulated Sox6.
Article in Cell & bioscience, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- Transcriptional dynamics of the murine heart during perinatal development at single-cell resolution.Biology open · 2026Article
- Understanding the Roles of Non-coding RNAs and Exosomal Non-Coding RNAs in Diabetic Nephropathy.Current molecular medicine · 2025Review
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- Transcriptome expression profiles associated with diabetic nephropathy development.Molecular and cellular biochemistry · 2022Article
- LncRNA H19 Regulates Proliferation, Apoptosis and ECM Degradation of Aortic Smooth Muscle Cells Via miR-1-3p/ADAM10 Axis in Thoracic Aortic Aneurysm.Biochemical genetics · 2022Article
- LncRNAs in Cardiomyocyte Maturation: New Window for Cardiac Regenerative Medicine.Non-coding RNA · 2021Review
- Non-coding RNAs in Cardiac Regeneration.Frontiers in physiology · 2021Review
- LncRNA-H19 Drives Cardiomyocyte Senescence by Targeting miR-19a/socs1/p53 Axis.Frontiers in pharmacology · 2021Article
- miRNA‑342 suppresses renal interstitial fibrosis in diabetic nephropathy by targeting SOX6.International journal of molecular medicine · 2020Article
- The long non-coding road to endogenous cardiac regeneration.Heart failure reviews · 2019Review
- LncRNA H19 promotes the committed differentiation of stem cells from apical papilla via miR-141/SPAG9 pathway.Cell death & disease · 2019Article
- Importance of Long Non-coding RNAs in the Development and Disease of Skeletal Muscle and Cardiovascular Lineages.Frontiers in cell and developmental biology · 2019Review
- The Relationship Between ZEB1-AS1 Expression and the Prognosis of Patients With Advanced Gastric Cancer Receiving Chemotherapy.Technology in cancer research & treatment · 2019Article
- The novel regulatory role of lncRNA-miRNA-mRNA axis in cardiovascular diseases.Journal of cellular and molecular medicine · 2018Review
- H19 Induces Abdominal Aortic Aneurysm Development and Progression.Circulation · 2018Article
- Long Non-Coding RNAs in Multifactorial Diseases: Another Layer of Complexity.Non-coding RNA · 2018Review
- miR-767-3p Inhibits Growth and Migration of Lung Adenocarcinoma Cells by Regulating CLDN18.Oncology research · 2018Article
- Non-coding RNAs: key regulators of smooth muscle cell fate in vascular disease.Cardiovascular research · 2018Review
- Lost in translation: The 3'-UTR ofEvolution, medicine, and public health · 2018Article
- PTENP1 inhibits the growth of esophageal squamous cell carcinoma by regulating SOCS6 expression and correlates with disease prognosis.Molecular carcinogenesis · 2017Article
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRegulating cardiac differentiation to maintain normal heart development and function is very important. At present, biological functions of H19 in cardiac differentiation is not completely clear.
methodsTo explore the functional effect of H19 during cardiac differentiation. Expression levels of early cardiac-specific markers Nkx-2.5 and GATA4, cardiac contractile protein genes α-MHC and MLC-2v were determined by qRT-PCR and western lot. The levels of lncRNA H19 and miR-19b were detected by qRT-PCR. We further predicted the binding sequence of H19 and miR-19b by online softwares starBase v2.0 and TargetScan. The biological functions of H19 and Sox6 were evaluated by CCK-8 kit, cell cycle and apoptosis assay and caspase-3 activity.
resultsThe expression levels of α-MHC, MLC-2v and H19 were upregulated, and miR-19b was downregulated significantly in mouse P19CL6 cells at the late stage of cardiac differentiation. Biological function analysis showed that knockdown of H19 promoted cell proliferation and inhibits cell apoptosis. H19 suppressed miR-19b expression and miR-19b targeted Sox6, which inhibited cell proliferation and promoted apoptosis in P19CL6 cells during late-stage cardiac differentiation. Importantly, Sox6 overexpression could reverse the positive effects of H19 knockdown on P19CL6 cells.
conclusionDownregulation of H19 promoted cell proliferation and inhibited cell apoptosis during late-stage cardiac differentiation by regulating the negative role of miR-19b in Sox6 expression, which suggested that the manipulation of H19 expression could serve as a potential strategy for heart disease.
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