ArticlePlant methods2016
A DNA-based real-time PCR assay for robust growth quantification of the bacterial pathogen
Article in Plant methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- The primary nitrate response TGA1 and TGA4 transcription factors are negative regulators of sulfate uptake and metabolism.Plant physiology · 2026Article
- Adaptation without Dominance in Pseudomonas syringae Pathovars.Microbial ecology · 2025Article
- Type III Secretion System-Mediated Induction of Systemic Resistance byPlants (Basel, Switzerland) · 2025Article
- Review
- Quantification of the fungal pathogen Didymella segeticola in Camellia sinensis using a DNA-based qRT-PCR assay.Plant methods · 2024Article
- AraDQ: an automated digital phenotyping software for quantifying disease symptoms of flood-inoculated Arabidopsis seedlings.Plant methods · 2024Article
- Nano-enabled crop resilience against pathogens: potential, mechanisms and strategies.Crop health · 2023Review
- Identifications of QTLs and Candidate Genes Associated withInternational journal of molecular sciences · 2023Article
- Arabidopsis CHROMATIN REMODELING 19 acts as a transcriptional repressor and contributes to plant pathogen resistance.The Plant cell · 2022Article
- Activation of plant immunity by exposure to dinitrogen pentoxide gas generated from air using plasma technology.PloS one · 2022Article
- Silica nanoparticles enhance disease resistance in Arabidopsis plants.Nature nanotechnology · 2021Article
- Agromonas: a rapid disease assay for Pseudomonas syringae growth in agroinfiltrated leaves.The Plant journal : for cell and molecular biology · 2021Article
- Benchmarking microbial growth rate predictions from metagenomes.The ISME journal · 2021Article
- Expression of a Fungal Lectin in Arabidopsis Enhances Plant Growth and Resistance Toward Microbial Pathogens and a Plant-Parasitic Nematode.Frontiers in plant science · 2021Article
- Engineering bacteriocin-mediated resistance against the plant pathogen Pseudomonas syringae.Plant biotechnology journal · 2020Article
- Transcriptional landscape of pathogen-responsive lncRNAs in rice unveils the role of ALEX1 in jasmonate pathway and disease resistance.Plant biotechnology journal · 2020Article
- Development of a DNA-based real-time PCR assay for the quantification ofPlant methods · 2020Article
- Reliable and early diagnosis of bacterial blight in pomegranate caused by Xanthomonas axonopodis pv. punicae using sensitive PCR techniques.Scientific reports · 2019Article
- Arabidopsis GCN2 kinase contributes to ABA homeostasis and stomatal immunity.Communications biology · 2019Article
- Evaluation of the RT-LAMP and LAMP methods for detection of Mycobacterium tuberculosis.Journal of clinical laboratory analysis · 2018Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe interaction of
resultsHere we present an alternative robust approach, which is based on the quantification of bacterial DNA by real-time PCR. We directly compared this assay with the routinely used plate counting method to access bacterial titers in a number of well described Arabidopsis mutants.
conclusionsThese studies showed that the DNA-based technique is highly reliable and comparable. Moreover, the technique is easily applicable, robust, and ideal for routine experiments or for larger scale analyses.
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