ArticlePlant physiology2017
A Palmitic Acid Elongase Affects Eicosapentaenoic Acid and Plastidial Monogalactosyldiacylglycerol Levels in Nannochloropsis.
Article in Plant physiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 82 citations in OpenAlex.
- The life cycle of fatty acids in oleaginous stramenopiles: from synthesis to maturation and beyond.BMC plant biology · 2026Review
- Decoupling of plastid and endomembrane homeoviscous response to low temperature and darkness in Phaeodactylum tricornutum.BMC plant biology · 2025Article
- Diatom triacylglycerol metabolism: from carbon fixation to lipid droplet degradation.Biological reviews of the Cambridge Philosophical Society · 2025Review
- Raman analysis of lipids in cells: Current applications and future prospects.Journal of pharmaceutical analysis · 2025Review
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- Monogalactosyldiacylglycerol synthase isoforms play diverse roles inside and outside the diatom plastid.The Plant cell · 2024Article
- Implicating the red body of Nannochloropsis in forming the recalcitrant cell wall polymer algaenan.Nature communications · 2024Article
- Characterization of Three Types of Elongases from Different Fungi and Site-Directed Mutagenesis.Journal of fungi (Basel, Switzerland) · 2024Article
- Review
- Growth and fatty acid distribution over lipid classes inFrontiers in plant science · 2023Article
- PDAT regulates PE as transient carbon sink alternative to triacylglycerol in Nannochloropsis.Plant physiology · 2022Article
- Synthesis of CInternational journal of molecular sciences · 2022Review
- Enhancement of violaxanthin accumulation inFrontiers in microbiology · 2022Article
- Comparative Proteomics Reveals Evidence of Enhanced EPA Trafficking in a Mutant Strain ofFrontiers in bioengineering and biotechnology · 2022Article
- Characterization of Nannochloropsis oceanica Rose Bengal Mutants Sheds Light on Acclimation Mechanisms to High Light When Grown in Low Temperature.Plant & cell physiology · 2021Article
- Plastidial acyl carrier protein Δ9-desaturase modulates eicosapentaenoic acid biosynthesis and triacylglycerol accumulation in Phaeodactylum tricornutum.The Plant journal : for cell and molecular biology · 2021Article
- Characterization of the Bubblegum acyl-CoA synthetase of Microchloropsis gaditana.Plant physiology · 2021Article
- Morphological bases of phytoplankton energy management and physiological responses unveiled by 3D subcellular imaging.Nature communications · 2021Article
- Grand Challenges in Microalgae Domestication.Frontiers in plant science · 2021Article
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Authors and funding
14 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nannochloropsis species are oleaginous eukaryotes containing a plastid limited by four membranes, deriving from a secondary endosymbiosis. In Nannochloropsis, thylakoid lipids, including monogalactosyldiacylglycerol (MGDG), are enriched in eicosapentaenoic acid (EPA). The need for EPA in MGDG is not understood. Fatty acids are de novo synthesized in the stroma, then converted into very-long-chain polyunsaturated fatty acids (FAs) at the endoplasmic reticulum (ER). The production of MGDG relies therefore on an EPA supply from the ER to the plastid, following an unknown process. We identified seven elongases and five desaturases possibly involved in EPA production in Nannochloropsis gaditana Among the six heterokont-specific saturated FA elongases possibly acting upstream in this pathway, we characterized the highly expressed isoform Δ0-ELO1 Heterologous expression in yeast (Saccharomyces cerevisiae) showed that NgΔ0-ELO1 could elongate palmitic acid. Nannochloropsis Δ0-elo1 mutants exhibited a reduced EPA level and a specific decrease in MGDG In NgΔ0-elo1 lines, the impairment of photosynthesis is consistent with a role of EPA-rich MGDG in nonphotochemical quenching control, possibly providing an appropriate MGDG platform for the xanthophyll cycle. Concomitantly with MGDG decrease, the level of triacylglycerol (TAG) containing medium chain FAs increased. In Nannochloropsis, part of EPA used for MGDG production is therefore biosynthesized by a channeled process initiated at the elongation step of palmitic acid by Δ0-ELO1, thus acting as a committing enzyme for galactolipid production. Based on the MGDG/TAG balance controlled by Δ0-ELO1, this study also provides novel prospects for the engineering of oleaginous microalgae for biotechnological applications.
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