ArticleAntimicrobial agents and chemotherapy2017
A Naturally Occurring Single Nucleotide Polymorphism in a Multicopy Plasmid Produces a Reversible Increase in Antibiotic Resistance.
Article in Antimicrobial agents and chemotherapy, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 43 citations in OpenAlex.
- The interplay between ecological networks drives host-plasmid community dynamics.PLoS computational biology · 2026Article
- Effectiveness of CRISPR-Cas in sensitizing bacterial populations with plasmid-encoded antimicrobial resistance.Genetics · 2025Article
- Universal rules govern plasmid copy number.Nature communications · 2025Article
- Evolutionary rescue of bacterial populations by heterozygosity on multicopy plasmids.Journal of mathematical biology · 2025Article
- Plasmid-mediated phenotypic noise leads to transient antibiotic resistance in bacteria.Nature communications · 2024Article
- Segregational drift hinders the evolution of antibiotic resistance on polyploid replicons.PLoS genetics · 2023Article
- A mathematician's guide to plasmids: an introduction to plasmid biology for modellers.Microbiology (Reading, England) · 2023Review
- Article
- Factors associated with plasmid antibiotic resistance gene carriage revealed using large-scale multivariable analysis.Scientific reports · 2023Article
- Article
- Evolution of ColE1-like plasmids across γ-Proteobacteria: From bacteriocin production to antimicrobial resistance.PLoS genetics · 2021Article
- Increased copy number couples the evolution of plasmid horizontal transmission and plasmid-encoded antibiotic resistance.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Beyond horizontal gene transfer: the role of plasmids in bacterial evolution.Nature reviews. Microbiology · 2021Review
- Extensive antimicrobial resistance mobilization via multicopy plasmid encapsidation mediated by temperate phages.The Journal of antimicrobial chemotherapy · 2020Article
- Article
- A High-Resolution View of Adaptive Event Dynamics in a Plasmid.Genome biology and evolution · 2019Article
- Article
- Lower Respiratory Tract Microbiome and Resistome of Bovine Respiratory Disease Mortalities.Microbial ecology · 2019Article
- PATRIC as a unique resource for studying antimicrobial resistance.Briefings in bioinformatics · 2019Article
- Segregational Drift and the Interplay between Plasmid Copy Number and Evolvability.Molecular biology and evolution · 2019Article
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Authors and funding
7 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
ColE1 plasmids are small mobilizable replicons that play an important role in the spread of antibiotic resistance in Pasteurellaceae In this study, we describe how a natural single nucleotide polymorphism (SNP) near the origin of replication of the ColE1-type plasmid pB1000 found in a Pasteurella multocida clinical isolate generates two independent plasmid variants able to coexist in the same cell simultaneously. Using the Haemophilus influenzae Rd KW20 strain as a model system, we combined antibiotic susceptibility tests, quantitative PCRs, competition assays, and experimental evolution to characterize the consequences of the coexistence of the pB1000 plasmid variants. This coexistence produced an increase of the total plasmid copy number (PCN) in the host bacteria, leading to a rise in both the antibiotic resistance level and the metabolic burden produced by pB1000. Using experimental evolution, we showed that in the presence of ampicillin, the bacteria maintained both plasmid variants for 300 generations. In the absence of antibiotics, on the other hand, the bacteria are capable of reverting to the single-plasmid genotype via the loss of one of the plasmid variants. Our results revealed how a single mutation in plasmid pB1000 provides the bacterial host with a mechanism to increase the PCN and, consequently, the ampicillin resistance level. Crucially, this mechanism can be rapidly reversed to avoid the extra cost entailed by the increased PCN in the absence of antibiotics.
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