ArticleParasites & vectors2016
Do mosquitoes transmit the avian malaria-like parasite Haemoproteus? An experimental test of vector competence using mosquito saliva.
Article in Parasites & vectors, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed, 45 citations in OpenAlex.
- Culex mosquitoes in a French Guiana zoo: insights on species diversity, feeding habits, and parasitic associations.Parasites & vectors · 2026Article
- Environmental factors affect the communities of avian Plasmodium in two species of Culex mosquitoes.Scientific reports · 2026Article
- Heterogeneous distribution of avian malaria parasites across mosquito species: disproportionate involvement of Culex restuans in the Midwestern United States.Malaria journal · 2026Article
- Oil palm expansion reshapes Culicoides assemblages and avian haemosporidian infections.Parasites & vectors · 2026Article
- Blood Parasites and Wildlife: The Development of a Discipline.Integrative zoology · 2026Review
- The lineage diversity, spatiotemporal distribution and pathological significance ofInternational journal for parasitology. Parasites and wildlife · 2025Article
- Effects of avian Plasmodium exposure on the microbiota of Culex pipiens.Scientific reports · 2025Article
- Combining blood meal analysis and parasite detection yields a more comprehensive understanding of insect host feeding patterns.Parasites & vectors · 2025Article
- Taking it with a grain of salt: tolerance to increasing salinization in Culex pipiens (Diptera: Culicidae) across a low-lying delta.Parasites & vectors · 2024Article
- High Abundance ofInsects · 2024Article
- Avian Plasmodium in invasive and native mosquitoes from southern Spain.Parasites & vectors · 2024Article
- Are the Culex pipiens biotypes pipiens, molestus and their hybrids competent vectors of avian Plasmodium?PloS one · 2024Article
- Avian Plasmodium spp. and Haemoproteus spp. parasites in mosquitoes in Germany.Parasites & vectors · 2023Article
- First evidence for development of Plasmodium relictum (Grassi and Feletti, 1891) sporozoites in the salivary glands of Culex modestus Ficalbi, 1889.Parasitology research · 2023Article
- Review
- Prevalence and intensity of avian malaria in a quail hybrid zone.Ecology and evolution · 2021Article
- Prevalence and Genetic Diversity of Avian Haemosporidian Parasites in Southern Iran.Pathogens (Basel, Switzerland) · 2021Article
- Assessing Diversity,Insects · 2021Article
- Mosquito identification and haemosporidian parasites detection in the enclosure of the African penguins (International journal for parasitology. Parasites and wildlife · 2020Article
- Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe life-cycle of many vector-borne pathogens includes an asexual replication phase in the vertebrate host and sexual reproduction in the insect vector. However, as only a small array of parasites can successfully develop infective phases inside an insect, few insect species are competent vectors for these pathogens. Molecular approaches have identified the potential insect vectors of blood parasites under natural conditions. However, the effectiveness of this methodology for verifying mosquito competence in the transmission of avian malaria parasites and related haemosporidians is still under debate. This is mainly because positive amplifications of parasite DNA in mosquitoes can be obtained not only from sporozoites, the infective phase of the malaria parasites that migrate to salivary glands, but also from different non-infective parasite forms in the body of the vector. Here, we assessed the vectorial capacity of the common mosquito Culex pipiens in the transmission of two parasite genera.
methodsA total of 1,560 mosquitoes were allowed to feed on five house sparrows Passer domesticus naturally infected by Haemoproteus or co-infected by Haemoproteus/Plasmodium. A saliva sample of the mosquitoes that survived after 13 days post-exposure was taken to determine the presence of parasite DNA by PCR.
resultsOverall, 31.2% mosquito's head-thorax and 5.8% saliva samples analysed showed positive amplifications for avian malaria parasites. In contrast to Haemoproteus DNA, which was not found in either the body parts or the saliva, Plasmodium DNA was detected in both the head-thorax and the saliva of mosquitoes. Parasites isolated from mosquitoes feeding on the same bird corresponded to the same Plasmodium lineage.
conclusionsOur experiment provides good evidence for the competence of Cx. pipiens in the transmission of Plasmodium but not of Haemoproteus. Molecular analyses of saliva are an effective method for testing the vector competence of mosquitoes and other insects in the transmission of vector-borne pathogens.
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