ArticlePloS one2016
Spatial Distribution of Selected Chemical Cell Wall Components in the Embryogenic Callus of Brachypodium distachyon.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 32 citations in OpenAlex.
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- Enrichment of 3D-Printed k-Carrageenan Food Gel with Callus Tissue of Narrow-Leaved LupinGels (Basel, Switzerland) · 2023Article
- One-Week Scutellar Somatic Embryogenesis in the MonocotPlants (Basel, Switzerland) · 2022Article
- 3,4-Dehydro-L-proline Induces Programmed Cell Death in the Roots ofInternational journal of molecular sciences · 2021Article
- Defining the Cell Wall, Cell Cycle and Chromatin Landmarks in the Responses ofInternational journal of molecular sciences · 2021Article
- Immunodetection of Pectic Epitopes, Arabinogalactan Proteins, and Extensins in Mucilage Cells from the Ovules ofInternational journal of molecular sciences · 2020Article
- Cell Wall Composition as a Marker of the Reprogramming of the Cell Fate on the Example of aInternational journal of molecular sciences · 2020Article
- From Single Cell to Plants: Mesophyll Protoplasts as a Versatile System for Investigating Plant Cell Reprogramming.International journal of molecular sciences · 2020Review
- Extracellular matrix and wall composition are diverse in the organogenic and non-organogenic calli of Actinidia arguta.Plant cell reports · 2020Article
- In Vitro Tissue Culture inInternational journal of molecular sciences · 2020Review
- Composition of the Reconstituted Cell Wall in Protoplast-Derived Cells of Daucus is Affected by Phytosulfokine (PSK).International journal of molecular sciences · 2019Article
- Hydroxyproline-Rich Glycoproteins as Markers of Temperature Stress in the Leaves ofInternational journal of molecular sciences · 2019Article
- Unmethyl-esterified homogalacturonan and extensins seal Arabidopsis graft union.BMC plant biology · 2019Article
- Cell Wall Epitopes and Endoploidy as Reporters of Embryogenic Potential inInternational journal of molecular sciences · 2018Article
- 5-Azacitidine Induces Cell Death in a Tissue Culture ofInternational journal of molecular sciences · 2018Article
- Organ and Tissue-Specific Localisation of Selected Cell Wall Epitopes in the Zygotic Embryo ofInternational journal of molecular sciences · 2018Article
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7 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Brachypodium distachyon L. Beauv. (Brachypodium) is a species that has become an excellent model system for gaining a better understanding of various areas of grass biology and improving plant breeding. Although there are some studies of an in vitro Brachypodium culture including somatic embryogenesis, detailed knowledge of the composition of the main cell wall components in the embryogenic callus in this species is missing. Therefore, using the immunocytochemical approach, we targeted 17 different antigens of which five were against the arabinogalactan proteins (AGP), three were against extensins, six recognised pectic epitopes and two recognised hemicelluloses. These studies were complemented by histological and scanning electron microscopy (SEM) analyses. We revealed that the characteristic cell wall components of Brachypodium embryogenic calli are AGP epitopes that are recognised by the JIM16 and LM2 antibodies, an extensin epitope that is recognised by the JIM11 antibody and a pectic epitopes that is recognised by the LM6 antibody. Furthermore, we demonstrated that AGPs and pectins are the components of the extracellular matrix network in Brachypodium embryogenic culture. Additionally, SEM analysis demonstrated the presence of an extracellular matrix on the surface of the calli cells. In conclusion, the chemical compositions of the cell walls and ECMSN of Brachypodium callus show spatial differences that correlate with the embryogenic character of the cells. Thus, the distribution of pectins, AGPs and hemicelluloses can be used as molecular markers of embryogenic cells. The presented data extends the knowledge about the chemical composition of the embryogenic callus cells of Brachypodium.
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