ArticlePloS one2016
Expression and Regulation of PIWIL-Proteins and PIWI-Interacting RNAs in Rheumatoid Arthritis.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 35 citations in OpenAlex.
- A Novel piRNA-Mediated Epigenetic Axis: piR-36241 Exacerbates Pulpitis by Silencing the Protective Receptor ADGRG2 in Human Dental Pulp Stem Cells.International endodontic journal · 2026Article
- PIWIL4 regulates gene expression and piRNA levels in RSV-infected airway epithelial cells.iScience · 2026Article
- Exosomes derived from stem cells from apical papilla reduce Th17 cell differentiation and hyposalivation in a murine model of Sjögren's disease.BMC oral health · 2026Article
- Blood-based epigenetic biomarkers in rheumatoid arthritis: current knowledge and future perspectives.Frontiers in immunology · 2026Review
- PIWIL4 regulates gene expression and piRNA levels in RSV-infected airway epithelial cells.bioRxiv : the preprint server for biology · 2025Article
- MambaCAttnGCN+: a comprehensive framework integrating MambaTextCNN, cross-attention and graph convolution network for piRNA-disease association prediction.Scientific reports · 2025Article
- Digital PCR-Based Assessment of Piwi-RNA PIR35982 as a Supporting Biomarker in Rheumatoid Arthritis.Journal of inflammation research · 2025Article
- piRNA associates with immune diseases.Cell communication and signaling : CCS · 2024Review
- Differential expression and correlation of immunoregulation related piRNA in rheumatoid arthritis.Frontiers in immunology · 2023Article
- The regulatory function of piRNA/PIWI complex in cancer and other human diseases: The role of DNA methylation.International journal of biological sciences · 2022Review
- piRNAQuest V.2: an updated resource for searching through the piRNAome of multiple species.RNA biology · 2022Article
- piRNA-36741 regulates BMP2-mediated osteoblast differentiation via METTL3 controlled m6A modification.Aging · 2021Article
- An old weapon with a new function: PIWI-interacting RNAs in neurodegenerative diseases.Translational neurodegeneration · 2021Review
- Role of PIWI-like 4 in modulating neuronal differentiation from human embryonal carcinoma cells.RNA biology · 2020Article
- Analysis of piRNA expression spectra in a non-alcoholic fatty liver disease mouse model induced by a methionine- and choline-deficient diet.Experimental and therapeutic medicine · 2020Article
- High levels of PIWI-interacting RNAs are present in the small RNA landscape of prostate epithelium from vitamin D clinical trial specimens.The Prostate · 2019Article
- The function of ncRNAs in rheumatic diseases.Epigenomics · 2019Review
- Next-generation sequencing reveals differentially expressed small noncoding RNAs in uterine leiomyoma.Fertility and sterility · 2018Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveThe PIWIL (P-element induced wimpy testis like protein) subfamily of argonaute proteins is essential for Piwi-interacting RNA (piRNA) biogenesis and their function to silence transposons during germ-line development. Here we explored their presence and regulation in rheumatoid arthritis (RA).
methodsThe expression of PIWIL genes in RA and osteoarthritis (OA) synovial tissues and synovial fibroblasts (SF) was analysed by Real-time PCR, immunofluorescence and Western blot. The expression of piRNAs was quantified by next generation small RNA sequencing (NGS). The regulation of PIWI/piRNAs, proliferation and methylation of LINE-1 after silencing of PIWIL genes were studied.
resultsPIWIL2 and 4 mRNA were similarly expressed in synovial tissues and SF from RA and OA patients. However, on the protein level only PIWIL4 was strongly expressed in SF. Using NGS up to 300 piRNAs were identified in all SF without significant differences in expression levels between RA and OASF. Of interest, the analysis of the co-expression of the detected piRNAs revealed a less tightly regulated pattern of piRNA-823, -4153 and -16659 expression in RASF. In RASF and OASF, stimulation with TNFα+IL1β/TLR-ligands further significantly increased the expression levels of PIWIL2 and 4 mRNA and piRNA-16659 was significantly (4-fold) induced upon Poly(I:C) stimulation. Silencing of PIWIL2/4 neither affect LINE-1 methylation/expression nor proliferation of RASF.
conclusionWe detected a new class of small regulatory RNAs (piRNAs) and their specific binding partners (PIWIL2/4) in synovial fibroblasts. The differential regulation of co-expression of piRNAs in RASF and the induction of piRNA/Piwi-proteins by innate immune stimulators suggest a role in inflammatory processes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.