ArticleScientific reports2016
Peptides Labeled with Pyridinium Salts for Sensitive Detection and Sequencing by Electrospray Tandem Mass Spectrometry.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 37 citations in OpenAlex.
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- Qualitative and Quantitative Mass Spectrometry in Salivary Metabolomics and Proteomics.Metabolites · 2023Review
- Catch, Modify and Analyze: Methods of Chemoselective Modification of Cysteine-Containing Peptides.Molecules (Basel, Switzerland) · 2022Review
- Approaches to Improve the Quantitation of Oxytocin in Human Serum by Mass Spectrometry.Frontiers in chemistry · 2022Article
- Peptide Charge Derivatization as a Tool for Early Detection of Preeclampsia by Mass Spectrometry-A Comparison with the ELISA Test.Molecules (Basel, Switzerland) · 2021Article
- Analysis of Fragmentation Pathways of Peptide Modified with Quaternary Ammonium and Phosphonium Group as Ionization Enhancers.Molecules (Basel, Switzerland) · 2021Article
- High-Resolution Mass Spectrometry-Based Approaches for the Detection and Quantification of Peptidase Activity in Plasma.Molecules (Basel, Switzerland) · 2020Article
- Detection of Podocin in Human Urine Sediment Samples by Charge Derivatization and LC-MS-MRM Method.International journal of molecular sciences · 2020Article
- Enrichment of Cysteine-Containing Peptide by On-Resin Capturing and Fixed Charge Tag Derivatization for Sensitive ESI-MS Detection.Molecules (Basel, Switzerland) · 2020Article
- Article
- Trends in the Design of New Isobaric Labeling Reagents for Quantitative Proteomics.Molecules (Basel, Switzerland) · 2019Review
- New ionization tags based on the structure of the 5-azoniaspiro[4.4]nonyl tag for a sensitive peptide sequencing by mass spectrometry.Analytical and bioanalytical chemistry · 2018Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mass spectrometric analysis of trace amounts of peptides may be problematic due to the insufficient ionization efficiency resulting in limited sensitivity. One of the possible ways to overcome this problem is the application of ionization enhancers. Herein we developed new ionization markers based on 2,4,6-triphenylpyridinium and 2,4,6-trimethylpyridinium salts. Using of inexpensive and commercially available pyrylium salt allows selective derivatization of primary amino groups, especially those sterically unhindered, such as ε-amino group of lysine. The 2,4,6-triphenylpyridinium modified peptides generate in MS/MS experiments an abundant protonated 2,4,6-triphenylpyridinium ion. This fragment is a promising reporter ion for the multiple reactions monitoring (MRM) analysis. In addition, the fixed positive charge of the pyridinium group enhances the ionization efficiency. Other advantages of the proposed ionization enhancers are the simplicity of derivatization of peptides and the possibility of convenient incorporation of isotopic labels into derivatized peptides.
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