ArticleScientific reports2016
Trans-translation is essential in the human pathogen Legionella pneumophila.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 28 citations in OpenAlex.
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- Physiology of trans-translation deficiency in Bacillus subtilis - a comparative proteomics study.Proteomics · 2023Article
- How hydrolytic exoribonucleases impact human disease: Two sides of the same story.FEBS open bio · 2023Review
- Identification and initial characterization of Hfq-associated sRNAs in Histophilus somni strain 2336.PloS one · 2023Article
- Article
- Extensive epigenetic modification with large-scale chromosomal and plasmid recombination characterise the Legionella longbeachae serogroup 1 genome.Scientific reports · 2022Article
- Peptide Aptamer PA3 Attenuates the Viability ofFrontiers in microbiology · 2022Article
- Synergistic bactericidal activities of tobramycin with ciprofloxacin and azithromycin against Klebsiella pneumoniae.The Journal of antibiotics · 2021Article
- Transposon Insertion Sequencing in a Clinical Isolate of Legionella pneumophila Identifies Essential Genes and Determinants of Natural Transformation.Journal of bacteriology · 2021Article
- Release factor-dependent ribosome rescue by BrfA in the Gram-positive bacterium Bacillus subtilis.Nature communications · 2019Article
- A Small-Molecule Inhibitor ofAntimicrobial agents and chemotherapy · 2019Article
- KKL-35 Exhibits Potent Antibiotic Activity against Legionella Species Independently ofAntimicrobial agents and chemotherapy · 2018Article
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2 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Trans-translation is a ubiquitous bacterial mechanism for ribosome rescue in the event of translation stalling. Although trans-translation is not essential in several bacterial species, it has been found essential for viability or virulence in a wide range of pathogens. We describe here that trans-translation is essential in the human pathogen Legionella pneumophila, the etiologic agent of Legionnaire's disease (LD), a severe form of nosocomial and community-acquired pneumonia. The ssrA gene coding for tmRNA, the key component of trans-translation, could not be deleted in L. pneumophila. To circumvent this and analyse the consequences of impaired trans-translation, we placed ssrA under the control of a chemical inducer. Phenotypes associated with the inhibition of ssrA expression include growth arrest in rich medium, hampered cell division, and hindered ability to infect eukaryotic cells (amoebae and human macrophages). LD is often associated with failure of antibiotic treatment and death (>10% of clinical cases). Decreasing tmRNA levels led to significantly higher sensitivity to ribosome-targeting antibiotics, including to erythromycin. We also detected a higher sensitivity to the transcription inhibitor rifampicin. Both antibiotics are recommended treatments for LD. Thus, interfering with trans-translation may not only halt the infection, but could also potentiate the recommended therapeutic treatments of LD.
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