ArticleMicrobial ecology2017
Whole-Genome Enrichment Provides Deep Insights into Vibrio cholerae Metagenome from an African River.
Article in Microbial ecology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 34 citations in OpenAlex.
- Hybridization capture sequencing formBio · 2025Article
- Evaluating methods for genome sequencing ofMicrobial genomics · 2025Article
- ToxigenicApplied and environmental microbiology · 2023Article
- Environmental Factors Influencing Occurrence of Vibrio parahaemolyticus and Vibrio vulnificus.Applied and environmental microbiology · 2023Article
- A Combination of Metagenomic and Cultivation Approaches Reveals Hypermutator Phenotypes within Vibrio cholerae-Infected Patients.mSystems · 2021Article
- Enrichment of low abundance DNA/RNA by oligonucleotide-clicked iron oxide nanoparticles.Scientific reports · 2021Article
- Aquatic reservoir of Vibrio cholerae in an African Great Lake assessed by large scale plankton sampling and ultrasensitive molecular methods.ISME communications · 2021Article
- Genetic insights into the dark matter of the mammalian gut microbiota through targeted genome reconstruction.Environmental microbiology · 2021Article
- Toward Accurate and Robust Environmental Surveillance Using Metagenomics.Frontiers in genetics · 2021Article
- Dynamics of the Pacific oyster pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment next-generation sequencing strategy.Environmental microbiology · 2019Article
- Uncovering Bifidobacteria via Targeted Sequencing of the Mammalian Gut Microbiota.Microorganisms · 2019Article
- Isolation of novel gut bifidobacteria using a combination of metagenomic and cultivation approaches.Genome biology · 2019Article
- Elucidating Waterborne Pathogen Presence and Aiding Source Apportionment in an Impaired Stream.Applied and environmental microbiology · 2018Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The detection and typing of Vibrio cholerae in natural aquatic environments encounter major methodological challenges related to the fact that the bacterium is often present in environmental matrices at very low abundance in nonculturable state. This study applied, for the first time to our knowledge, a whole-genome enrichment (WGE) and next-generation sequencing (NGS) approach for direct genotyping and metagenomic analysis of low abundant V. cholerae DNA (<50 genome unit/L) from natural water collected in the Morogoro river (Tanzania). The protocol is based on the use of biotinylated RNA baits for target enrichment of V. cholerae metagenomic DNA via hybridization. An enriched V. cholerae metagenome library was generated and sequenced on an Illumina MiSeq platform. Up to 1.8 × 10
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.