Evidence map›Paper›PMID 27888074›Full record

ArticleBiochimica et biophysica acta. Proteins and proteomics2017

Toxoplasma gondii immune mapped protein 1 is anchored to the inner leaflet of the plasma membrane and adopts a novel protein fold.

Yonggen Jia, Stefi Benjamin, Qun Liu, Yingqi Xu, Sunil Kumar Dogga, Jing Liu, Stephen Matthews, Dominique Soldati-Favre

Erratum issuedOpen access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochimica et biophysica acta. Proteins and proteomics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.6field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 3 countries.

Yonggen JiaDepartment of Microbiology and Molecular Medicine, CMU, University of Geneva, Rue Michel-Servet 1, CH-1211 Geneva, Switzerland.
Stefi BenjaminDepartment of Life Sciences, Imperial College London, South Kensington, London SW7 2AZ, UK.
Qun LiuKey Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Yingqi XuDepartment of Life Sciences, Imperial College London, South Kensington, London SW7 2AZ, UK.
Sunil Kumar DoggaDepartment of Microbiology and Molecular Medicine, CMU, University of Geneva, Rue Michel-Servet 1, CH-1211 Geneva, Switzerland.
Jing LiuKey Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Stephen MatthewsDepartment of Life Sciences, Imperial College London, South Kensington, London SW7 2AZ, UK. Electronic address: s.j.matthews@imperial.ac.uk.
Dominique Soldati-FavreDepartment of Microbiology and Molecular Medicine, CMU, University of Geneva, Rue Michel-Servet 1, CH-1211 Geneva, Switzerland. Electronic address: dominique.soldati-Favre@unige.ch.
Imperial College London · GBUniversity of Geneva · CHChina Agricultural University · CN

Funding

Medical Research Council MR/J006874/1Wellcome TrustWellcome Trust 100280
6 · The paper itself

Abstract

The immune mapped protein 1 (IMP1) was first identified as a protective antigen in Eimeria maxima and described as vaccine candidate and invasion factor in Toxoplasma gondii. We show here that TgIMP1 localizes to the inner leaflet of plasma membrane (PM) via dual acylation. Mutations either in the N-terminal myristoylation or palmitoylation sites (G2 and C5) cause relocalization of TgIMP1 to the cytosol. The first 11 amino acids are sufficient for PM targeting and the presence of lysine (K7) is critical. Disruption of TgIMP1 gene by double homologous recombination revealed no invasion defect or any measurable alteration in the lytic cycle of tachyzoites. Following immunization with TgIMP1 DNA vaccine, mice challenged with either wild type or IMP1-ko parasites showed no significant difference in protection. The sequence analysis identified a structured C-terminal domain that is present in a broader family of IMP1-like proteins conserved across the members of Apicomplexa. We present the solution structure of this domain determined from NMR data and describe a new protein fold not seen before.

Indexed as

Amino Acid SequenceAntigens, ProtozoanCell MembraneCells, CulturedCytokinesHumansImmunizationProtozoan ProteinsRNA-Binding ProteinsToxoplasmaVaccinationVaccines, DNAAntigens, ProtozoanCytokinesimmune mapped protein 1, Toxoplasma gondiiProtozoan ProteinsRNA-Binding ProteinsVaccines, DNAAcylationInvasionNMRProtein structureToxoplasma gondiiVaccine

Identifiers

PMID27888074
PMCPMC5716462
OpenAlexW2556466344

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.