ArticleScientific reports2016
Time-lapse 3-D measurements of a glucose biosensor in multicellular spheroids by light sheet fluorescence microscopy in commercial 96-well plates.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 69 citations in OpenAlex.
- Serial Imaging of Tumour and microEnvironment (SITE) platform for live-cellbioRxiv : the preprint server for biology · 2025Article
- Open-top multisample dual-view light-sheet microscope for live imaging of large multicellular systems.Nature methods · 2024Article
- Light sheet illumination in single-molecule localization microscopy for imaging of cellular architectures and molecular dynamics.Npj imaging · 2024Review
- High-Throughput, Low Background, and Wide-Field Microscopy by Flat-Field Photobleaching Imprinting Microscopy.Chemical & biomedical imaging · 2023Article
- Recent advances in oblique plane microscopy.Nanophotonics (Berlin, Germany) · 2023Review
- Article
- Increasing the field-of-view in oblique plane microscopy via optical tiling.Biomedical optics express · 2022Article
- Multiscale light-sheet organoid imaging framework.Nature communications · 2022Article
- Automated high-speed 3D imaging of organoid cultures with multi-scale phenotypic quantification.Nature methods · 2022Article
- Lasers in Live Cell Microscopy.International journal of molecular sciences · 2022Review
- DaXi-high-resolution, large imaging volume and multi-view single-objective light-sheet microscopy.Nature methods · 2022Article
- Biosensors to Monitor Cell Activity in 3D Hydrogel-Based Tissue Models.Sensors (Basel, Switzerland) · 2022Review
- miniSPIM-A Miniaturized Light-Sheet Microscope.ACS sensors · 2021Article
- 3D-printed microplate inserts for long term high-resolution imaging of live brain organoids.BMC biomedical engineering · 2021Article
- Light Sheet Illumination for 3D Single-Molecule Super-Resolution Imaging of Neuronal Synapses.Frontiers in synaptic neuroscience · 2021Review
- Dual-view oblique plane microscopy (dOPM).Biomedical optics express · 2020Article
- L-Glucose: Another Path to Cancer Cells.Cancers · 2020Review
- Time-lapse imaging of HeLa spheroids in soft agar culture provides virtual inner proliferative activity.PloS one · 2020Article
- Probing pluripotency gene regulatory networks with quantitative live cell imaging.Computational and structural biotechnology journal · 2020Review
- Epi-illumination SPIM for volumetric imaging with high spatial-temporal resolution.Nature methods · 2019Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Light sheet fluorescence microscopy has previously been demonstrated on a commercially available inverted fluorescence microscope frame using the method of oblique plane microscopy (OPM). In this paper, OPM is adapted to allow time-lapse 3-D imaging of 3-D biological cultures in commercially available glass-bottomed 96-well plates using a stage-scanning OPM approach (ssOPM). Time-lapse 3-D imaging of multicellular spheroids expressing a glucose Förster resonance energy transfer (FRET) biosensor is demonstrated in 16 fields of view with image acquisition at 10 minute intervals. As a proof-of-principle, the ssOPM system is also used to acquire a dose response curve with the concentration of glucose in the culture medium being varied across 42 wells of a 96-well plate with the whole acquisition taking 9 min. The 3-D image data enable the FRET ratio to be measured as a function of distance from the surface of the spheroid. Overall, the results demonstrate the capability of the OPM system to measure spatio-temporal changes in FRET ratio in 3-D in multicellular spheroids over time in a multi-well plate format.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.