ArticlePlant biotechnology journal2017
Selective gene dosage by CRISPR-Cas9 genome editing in hexaploid Camelina sativa.
Article in Plant biotechnology journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 114 papers.
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Who cites it
114 citing papers in PubMed, 280 citations in OpenAlex.
- A haplotype-resolved chromosome-level genome assembly of autotetraploid Chinese yam (Horticulture research · 2026Article
- Winning by Losing: Exploiting Modified Plant Susceptibility Genes to Counteract Necrotrophic Fungal Pathogens.Plant biotechnology journal · 2025Review
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- The Potential of NGTs to Overcome Constraints in Plant Breeding and Their Regulatory Implications.International journal of molecular sciences · 2025Review
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- Complexity Meets Risk-The Next Generation of Genome-Edited Plants Challenges Established Concepts for Environmental Risk Assessment in the EU.Plants (Basel, Switzerland) · 2025Review
- Direct and indirect effects of multiplex genome editing of F5H and FAD2 in oil crop camelina.Plant biotechnology journal · 2025Article
- Ectopic Gene Conversion Causing Quantitative Trait Variation.Molecular biology and evolution · 2025Article
- Metabolic engineering of narrow-leafed lupin for the production of enantiomerically pure (-)-sparteine.Plant biotechnology journal · 2025Article
- Refining polyploid breeding in sweet potato through allele dosage enhancement.Nature plants · 2025Article
- Identification of novel genes responsible for a pollen killer present in local natural populations of Arabidopsis thaliana.PLoS genetics · 2025Article
- Genome-wide identification and diversity of FAD2, FAD3 and FAE1 genes in terms of biotechnological importance in Camelina species.BMC biotechnology · 2024Article
- Integration of CRISPR/Cas9 with multi-omics technologies to engineer secondary metabolite productions in medicinal plant: Challenges and Prospects.Functional & integrative genomics · 2024Review
- Retrotransposon-driven environmental regulation of FLC leads to adaptive response to herbicide.Nature plants · 2024Article
- Review
- Creating yellow seed Camelina sativa with enhanced oil accumulation by CRISPR-mediated disruption of Transparent Testa 8.Plant biotechnology journal · 2024Article
- Application of genome editing techniques to regulate gene expression in crops.BMC plant biology · 2024Review
- Improving linolenic acid content in rapeseed oil by overexpression ofMolecular breeding : new strategies in plant improvement · 2024Article
- Improvement of crop production in controlled environment agriculture through breeding.Frontiers in plant science · 2024Review
- Genome-wide evaluation of gene editing outcomes using CRISPR/Cas9 in seed propagatedFrontiers in plant science · 2024Article
54 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In many plant species, gene dosage is an important cause of phenotype variation. Engineering gene dosage, particularly in polyploid genomes, would provide an efficient tool for plant breeding. The hexaploid oilseed crop Camelina sativa, which has three closely related expressed subgenomes, is an ideal species for investigation of the possibility of creating a large collection of combinatorial mutants. Selective, targeted mutagenesis of the three delta-12-desaturase (FAD2) genes was achieved by CRISPR-Cas9 gene editing, leading to reduced levels of polyunsaturated fatty acids and increased accumulation of oleic acid in the oil. Analysis of mutations over four generations demonstrated the presence of a large variety of heritable mutations in the three isologous CsFAD2 genes. The different combinations of single, double and triple mutants in the T3 generation were isolated, and the complete loss-of-function mutants revealed the importance of delta-12-desaturation for Camelina development. Combinatorial association of different alleles for the three FAD2 loci provided a large diversity of Camelina lines with various lipid profiles, ranging from 10% to 62% oleic acid accumulation in the oil. The different allelic combinations allowed an unbiased analysis of gene dosage and function in this hexaploid species, but also provided a unique source of genetic variability for plant breeding.
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