Evidence map›Paper›PMID 27882487›Full record

ArticleThe AAPS journal2017

Delivery of Small Interfering RNA to Inhibit Vascular Endothelial Growth Factor in Zebrafish Using Natural Brain Endothelia Cell-Secreted Exosome Nanovesicles for the Treatment of Brain Cancer.

Tianzhi Yang, Brittany Fogarty, Bret LaForge, Salma Aziz, Thuy Pham, Leanne Lai, Shuhua Bai

Abstract read
PubMed Publisher
In one paragraph

Article in The AAPS journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 105 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
105citing papers in PubMed, 1 pooled it
5.6field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

105 citing papers in PubMed, 1 synthesis or guideline pooled it, 192 citations in OpenAlex.

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  18. Glioma-Derived Exosomes and Their Application as Drug Nanoparticles.International journal of molecular sciences · 2024
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45 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Tianzhi YangDepartment of Basic Pharmaceutical Sciences, School of Pharmacy, Husson University, Bangor, Maine, 04401, USA.
Brittany FogartyDepartment of Basic Pharmaceutical Sciences, School of Pharmacy, Husson University, Bangor, Maine, 04401, USA.
Bret LaForgeDepartment of Basic Pharmaceutical Sciences, School of Pharmacy, Husson University, Bangor, Maine, 04401, USA.
Salma AzizDepartment of Basic Pharmaceutical Sciences, School of Pharmacy, Husson University, Bangor, Maine, 04401, USA.
Thuy PhamDepartment of Basic Pharmaceutical Sciences, School of Pharmacy, Husson University, Bangor, Maine, 04401, USA.
Leanne LaiDepartment of Sociobehavioral and Administrative Pharmacy, College of Pharmacy, Nova Southeastern University, Ft. Lauderdale, Florida, 33328, USA.
Shuhua BaiDepartment of Basic Pharmaceutical Sciences, School of Pharmacy, Husson University, Bangor, Maine, 04401, USA. BaiS@husson.edu.
Husson University · USNova Southeastern University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Although small interfering RNA (siRNA) holds great therapeutic promise, its delivery to the disease site remains a paramount obstacle. In this study, we tested whether brain endothelial cell-derived exosomes could deliver siRNA across the blood-brain barrier (BBB) in zebrafish. Natural exosomes were isolated from brain endothelial bEND.3 cell culture media and vascular endothelial growth factor (VEGF) siRNA was loaded in exosomes with the assistance of a transfection reagent. While fluorescence-activated cell flow cytometry and immunocytochemistry staining studies indicated that wild-type exosomes significantly increased the uptake of fluorescence-labeled siRNA in the autologous brain endothelial cells, decreased fluorescence intensity was observed in the cells treated with the tetraspanin CD63 antibody-blocked exosome-delivered formulation (p < 0.05). In the transport study, exosomes also enhanced the permeability of rhodamine 123 in a co-cultured monolayer of brain endothelial bEND.3 cell and astrocyte. Inhibition at the expression of VEGF RNA and protein levels was observed in glioblastoma-astrocytoma U-87 MG cells treated with exosome-delivered siRNAs. Imaging results showed that exosome delivered more siRNAs across the BBB in Tg(fli1:GFP) zebrafish. In a xenotransplanted brain tumor model, exosome-delivered VEGF siRNAs decreased the fluorescence intensity of labeled cancer cells in the brain of zebrafish. Brain endothelial cell-derived exosomes could be potentially used as a natural carrier for the brain delivery of exogenous siRNA.

Indexed as

Gene Transfer TechniquesAnimalsAstrocytesBlood-Brain BarrierBrainBrain NeoplasmsCell Line, TumorCoculture TechniquesEndothelial CellsExosomesHumansRNA, Small InterferingTransfectionVascular Endothelial Growth Factor AZebrafishRNA, Small InterferingVascular Endothelial Growth Factor Ablood–brain barrierbrain tumor therapyexosomesiRNA

Identifiers

PMID27882487
OpenAlexW2552099798

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.