Evidence map›Paper›PMID 27882063›Full record

ArticleHepatitis monthly2016

Design, Construction and Evaluation of 1a/JFH1 HCV Chimera by Replacing the Intergenotypic Variable Region.

Faezeh Ghasemi, Majid Ghayour-Mobarhan, Alireza Pasdar, Hamid Pourianfar, Mohammad Reza Aghasadeghi, Hamed Gouklani, Zahra Meshkat

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Article in Hepatitis monthly, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Faezeh GhasemiDepartment of Medical Biotechnology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IR Iran.
Majid Ghayour-MobarhanBiochemistry of Nutrition Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IR Iran.
Alireza PasdarFaculty of Medicine, Department of New Sciences and Technology, Mashhad University of Medical Sciences, Mashhad, IR Iran; Division of Applied Medicine, Medical School, University of Aberdeen, Foresterhill, Aberdeen, UK.
Hamid PourianfarIndustrial Biotechnology Research Institute, Iranian Academic Centre for Education, Culture and Research Mashhad Branch, Mashhad, IR Iran.
Mohammad Reza AghasadeghiHepatitis and AIDS Department, Pasteur Institute of Iran, Tehran, IR Iran.
Hamed GouklaniMolecular Medicine Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, IR Iran.
Zahra MeshkatAntimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, IR Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe E2 glycoprotein is an important encoded hepatitis C virus (HCV) protein that contains three different variable regions.

objectivesThe aim of the present study was to construct an HCV 1a/JFH1 chimeric virus by replacing the intergenotypic variable region (igVR) fragment of the highly variable region of the E2 gene of the Japanese Fulminant hepatitis genotype 2a JFH1 virus with a similar region of HCV genotype 1a. This chimera was produced as a model virus with the ability to be cultured. We analyzed the adapted virus and the variations of nucleic acids within it.

methodsSpecific primers were designed for the igVR of HCV genotype 1a followed by the overlap-PCR method for the synthesis of the desired DNA fragment. The amplified igVR-1a chimera gene and pFL-J6/JFH were digested by

resultsQuantitative real-time PCR results showed 11,292,514 copies/mL of chimeric virus production in cell culture. The virus production was confirmed using ELISA, which showed a virus core production of 808.2 pg/mL. The results of cloning and sequencing showed that some of the nucleic acids in the chimera virus were changed, affecting the viral behavior in the cell culture.

conclusionsReal-time PCR and ELISA showed high levels of production of 1a/JFH1 chimeric HCV in the Huh7.5 cell culture. The constructed virus can be used for future studies, including the development of new HCV drugs and vaccines.

Indexed as

E2 GlycoproteinHepatitis C VirusIntergenotypic Variable RegionReal-time PCR

Identifiers

PMID27882063
PMCPMC5111421

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