ArticlePlant physiology2017
A Tightly Regulated Genetic Selection System with Signaling-Active Alleles of Phytochrome B.
Article in Plant physiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 9 citations in OpenAlex.
- Intragenic suppressor screen of YHB identifies novel and known loss-of-function alleles of Arabidopsis phytochrome B.bioRxiv : the preprint server for biology · 2026Article
- A cytosol-tethered YHB variant of phytochrome B retains photomorphogenic signaling activity.Plant molecular biology · 2024Article
- Direct photoresponsive inhibition of a p53-like transcription activation domain in PIF3 by Arabidopsis phytochrome B.Nature communications · 2021Article
- Regulation of monocot and dicot plant development with constitutively active alleles of phytochrome B.Plant direct · 2020Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
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Abstract
Selectable markers derived from plant genes circumvent the potential risk of antibiotic/herbicide-resistance gene transfer into neighboring plant species, endophytic bacteria, and mycorrhizal fungi. Toward this goal, we have engineered and validated signaling-active alleles of phytochrome B (eYHB) as plant-derived selection marker genes in the model plant Arabidopsis (Arabidopsis thaliana). By probing the relationship of construct size and induction conditions to optimal phenotypic selection, we show that eYHB-based alleles are robust substitutes for antibiotic/herbicide-dependent marker genes as well as surprisingly sensitive reporters of off-target transgene expression.
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