ArticlePhysiological reports2016
Intestinal Na+, K+, 2Cl- cotransporter 2 plays a crucial role in hyperosmotic transitions of a euryhaline teleost.
Article in Physiological reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
- Comparative Evolution of SLC12 Transporters in Tilapias and Salinity-Responsive Expression in Nile Tilapia.International journal of molecular sciences · 2026Article
- Adaptation responses to salt stress in the gut ofAnimal cells and systems · 2025Article
- The role of environmental salinity on NaScientific reports · 2022Article
- NaJournal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2022Article
- Integrated Omics Approaches Revealed the Osmotic Stress-Responsive Genes and Microbiota in Gill of Marine Medaka.mSystems · 2022Article
- Tissue and salinity specific NaScientific reports · 2021Article
- Osmoregulatory plasticity during hypersaline acclimation in red drum, Sciaenops ocellatus.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2021Article
- The digestive tract as an essential organ for water acquisition in marine teleosts: lessons from euryhaline eels.Zoological letters · 2021Review
- Ion Transporters and Osmoregulation in the Kidney of Teleost Fishes as a Function of Salinity.Frontiers in physiology · 2021Review
- Immunological characterization of two types of ionocytes in the inner ear epithelium of Pacific Chub Mackerel (Scomber japonicus).Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2020Article
- Osmoregulation in the Plotosidae Catfish: Role of the Salt Secreting Dendritic Organ.Frontiers in physiology · 2018Article
- Acclimation to prolonged hypoxia alters hemoglobin isoform expression and increases hemoglobin oxygen affinity and aerobic performance in a marine fish.Scientific reports · 2017Article
- Carbon dioxide induced plasticity of branchial acid-base pathways in an estuarine teleost.Scientific reports · 2017Article
- Intestinal Na+, K+, 2Cl- cotransporter 2 plays a crucial role in hyperosmotic transitions of a euryhaline teleost.Physiological reports · 2016Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Euryhaline fishes, such as the red drum (Sciaenops ocellatus), must quickly transition between hyperosmotic and hypoosmotic physiological strategies. When freshwater individuals transition to seawater they are exposed to increased diffusive water loss and ion gain. To maintain osmoregulatory balance these animals must drink and absorb seawater through the intestine, followed by ion excretion at the gills. The ability of fishes to transition between strategies can limit the magnitude of osmotic shock that can be tolerated. Here, we demonstrate that red drum can tolerate direct transfer from freshwater to full-strength seawater with marginal impacts on osmotic balance, as indicated by plasma and muscle ion concentration, as well as muscle water. Seawater transition is concurrent with a significant increase in intestinal fluid volume. Typical patterns of osmoregulatory plasticity were observed in the gill with increased expression of nkcc1 and cftr Expression changes in the anterior intestine were observed after 24 h for nkcc2 with smaller and later responses observed for slc26a3, slc26a6, and nbc Immunofluorescence staining demonstrated similar patterns of NKCC localization in freshwater and seawater intestines; however, reduced basolateral staining of V-type ATPase was observed in seawater. Electrophysiological preparations demonstrated that seawater fish had increased absorptive current in the anterior intestine, which was significantly reduced in the presence of 10 μmol/L bumetanide. Overall, these results suggest that nkcc2 plays a crucial role during hyperosmotic transitions, and may be a more important complement to the well-known bicarbonate secretion pathway than generally considered.
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