Evidence map›Paper›PMID 27881556›Full record

Trial reportCirculation2017

Nitrite and S-Nitrosohemoglobin Exchange Across the Human Cerebral and Femoral Circulation: Relationship to Basal and Exercise Blood Flow Responses to Hypoxia.

Damian M Bailey, Peter Rasmussen, Morten Overgaard, Kevin A Evans, Aske M Bohm, Thomas Seifert, Patrice Brassard, Morten Zaar, Henning B Nielsen, Peter B Raven and 1 more

Open access · bronzeAbstract readRandomized Controlled Trial
PubMed Publisher
In one paragraph

Trial report in Circulation, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed, 2 pooled it
4.1field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 2 syntheses or guidelines pooled it, 66 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
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  7. Review
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  12. Hemoglobin and cerebral hypoxic vasodilation in humans: Evidence for nitric oxide-dependent andJournal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2023
    Article
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  15. Review
  16. Cerebral OJournal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2023
    Article
  17. Review
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Damian M BaileyFrom Faculty of Medicine, Reichwald Health Sciences Centre, University of British Columbia-Okanagan, Kelowna, BC, Canada (D.M.B.); Neurovascular Research Laboratory, Faculty of Life Sciences and Education, University of South Wales, Glamorgan, UK (K.A.E.); Department of Anesthesia, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark (P.R., M.D., A.M.B., T.S., P.B., M.Z., H.B.N., N.H.S.); Université Laval, Québec, QC, Canada (P.B.); and Department of Integrative Physiology and Anatomy, University of North Texas, Health Science Center, Ft Worth (P.B.R.). damian.bailey@southwales.ac.uk.
Peter RasmussenFrom Faculty of Medicine, Reichwald Health Sciences Centre, University of British Columbia-Okanagan, Kelowna, BC, Canada (D.M.B.); Neurovascular Research Laboratory, Faculty of Life Sciences and Education, University of South Wales, Glamorgan, UK (K.A.E.); Department of Anesthesia, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark (P.R., M.D., A.M.B., T.S., P.B., M.Z., H.B.N., N.H.S.); Université Laval, Québec, QC, Canada (P.B.); and Department of Integrative Physiology and Anatomy, University of North Texas, Health Science Center, Ft Worth (P.B.R.).
Morten OvergaardFrom Faculty of Medicine, Reichwald Health Sciences Centre, University of British Columbia-Okanagan, Kelowna, BC, Canada (D.M.B.); Neurovascular Research Laboratory, Faculty of Life Sciences and Education, University of South Wales, Glamorgan, UK (K.A.E.); Department of Anesthesia, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark (P.R., M.D., A.M.B., T.S., P.B., M.Z., H.B.N., N.H.S.); Université Laval, Québec, QC, Canada (P.B.); and Department of Integrative Physiology and Anatomy, University of North Texas, Health Science Center, Ft Worth (P.B.R.).
Kevin A EvansFrom Faculty of Medicine, Reichwald Health Sciences Centre, University of British Columbia-Okanagan, Kelowna, BC, Canada (D.M.B.); Neurovascular Research Laboratory, Faculty of Life Sciences and Education, University of South Wales, Glamorgan, UK (K.A.E.); Department of Anesthesia, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark (P.R., M.D., A.M.B., T.S., P.B., M.Z., H.B.N., N.H.S.); Université Laval, Québec, QC, Canada (P.B.); and Department of Integrative Physiology and Anatomy, University of North Texas, Health Science Center, Ft Worth (P.B.R.).
Aske M BohmFrom Faculty of Medicine, Reichwald Health Sciences Centre, University of British Columbia-Okanagan, Kelowna, BC, Canada (D.M.B.); Neurovascular Research Laboratory, Faculty of Life Sciences and Education, University of South Wales, Glamorgan, UK (K.A.E.); Department of Anesthesia, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark (P.R., M.D., A.M.B., T.S., P.B., M.Z., H.B.N., N.H.S.); Université Laval, Québec, QC, Canada (P.B.); and Department of Integrative Physiology and Anatomy, University of North Texas, Health Science Center, Ft Worth (P.B.R.).
Thomas SeifertFrom Faculty of Medicine, Reichwald Health Sciences Centre, University of British Columbia-Okanagan, Kelowna, BC, Canada (D.M.B.); Neurovascular Research Laboratory, Faculty of Life Sciences and Education, University of South Wales, Glamorgan, UK (K.A.E.); Department of Anesthesia, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark (P.R., M.D., A.M.B., T.S., P.B., M.Z., H.B.N., N.H.S.); Université Laval, Québec, QC, Canada (P.B.); and Department of Integrative Physiology and Anatomy, University of North Texas, Health Science Center, Ft Worth (P.B.R.).
Patrice BrassardFrom Faculty of Medicine, Reichwald Health Sciences Centre, University of British Columbia-Okanagan, Kelowna, BC, Canada (D.M.B.); Neurovascular Research Laboratory, Faculty of Life Sciences and Education, University of South Wales, Glamorgan, UK (K.A.E.); Department of Anesthesia, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark (P.R., M.D., A.M.B., T.S., P.B., M.Z., H.B.N., N.H.S.); Université Laval, Québec, QC, Canada (P.B.); and Department of Integrative Physiology and Anatomy, University of North Texas, Health Science Center, Ft Worth (P.B.R.).
Morten ZaarFrom Faculty of Medicine, Reichwald Health Sciences Centre, University of British Columbia-Okanagan, Kelowna, BC, Canada (D.M.B.); Neurovascular Research Laboratory, Faculty of Life Sciences and Education, University of South Wales, Glamorgan, UK (K.A.E.); Department of Anesthesia, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark (P.R., M.D., A.M.B., T.S., P.B., M.Z., H.B.N., N.H.S.); Université Laval, Québec, QC, Canada (P.B.); and Department of Integrative Physiology and Anatomy, University of North Texas, Health Science Center, Ft Worth (P.B.R.).
Henning B NielsenFrom Faculty of Medicine, Reichwald Health Sciences Centre, University of British Columbia-Okanagan, Kelowna, BC, Canada (D.M.B.); Neurovascular Research Laboratory, Faculty of Life Sciences and Education, University of South Wales, Glamorgan, UK (K.A.E.); Department of Anesthesia, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark (P.R., M.D., A.M.B., T.S., P.B., M.Z., H.B.N., N.H.S.); Université Laval, Québec, QC, Canada (P.B.); and Department of Integrative Physiology and Anatomy, University of North Texas, Health Science Center, Ft Worth (P.B.R.).
Peter B RavenFrom Faculty of Medicine, Reichwald Health Sciences Centre, University of British Columbia-Okanagan, Kelowna, BC, Canada (D.M.B.); Neurovascular Research Laboratory, Faculty of Life Sciences and Education, University of South Wales, Glamorgan, UK (K.A.E.); Department of Anesthesia, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark (P.R., M.D., A.M.B., T.S., P.B., M.Z., H.B.N., N.H.S.); Université Laval, Québec, QC, Canada (P.B.); and Department of Integrative Physiology and Anatomy, University of North Texas, Health Science Center, Ft Worth (P.B.R.).
Niels H SecherFrom Faculty of Medicine, Reichwald Health Sciences Centre, University of British Columbia-Okanagan, Kelowna, BC, Canada (D.M.B.); Neurovascular Research Laboratory, Faculty of Life Sciences and Education, University of South Wales, Glamorgan, UK (K.A.E.); Department of Anesthesia, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark (P.R., M.D., A.M.B., T.S., P.B., M.Z., H.B.N., N.H.S.); Université Laval, Québec, QC, Canada (P.B.); and Department of Integrative Physiology and Anatomy, University of North Texas, Health Science Center, Ft Worth (P.B.R.).
University of South Wales · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe mechanisms underlying red blood cell (RBC)-mediated hypoxic vasodilation remain controversial, with separate roles for nitrite () and S-nitrosohemoglobin (SNO-Hb) widely contested given their ability to transduce nitric oxide bioactivity within the microcirculation. To establish their relative contribution in vivo, we quantified arterial-venous concentration gradients across the human cerebral and femoral circulation at rest and during exercise, an ideal model system characterized by physiological extremes of O

methodsTen healthy participants (5 men, 5 women) aged 24±4 (mean±SD) years old were randomly assigned to a normoxic (21% O

resultsHypoxia was associated with a mild increase in both cerebral blood flow and femoral blood flow (P<0.05 versus normoxia) with further, more pronounced increases observed in femoral blood flow during exercise (P<0.05 versus rest) in proportion to the reduction in RBC oxygenation (r=0.680-0.769, P<0.001). Plasma gradients reflecting consumption (arterial>venous; P<0.05) were accompanied by RBC iron nitrosylhemoglobin formation (venous>arterial; P<0.05) at rest in normoxia, during hypoxia (P<0.05 versus normoxia), and especially during exercise (P<0.05 versus rest), with the most pronounced gradients observed across the bioenergetically more active, hypoxemic, and acidotic femoral circulation (P<0.05 versus cerebral). In contrast, we failed to observe any gradients consistent with RBC SNO-Hb consumption and corresponding delivery of plasma S-nitrosothiols (P>0.05).

conclusionsThese findings suggest that hypoxia and, to a far greater extent, exercise independently promote arterial-venous delivery gradients of intravascular nitric oxide, with deoxyhemoglobin-mediated reduction identified as the dominant mechanism underlying hypoxic vasodilation.

Indexed as

AdultCerebrovascular CirculationErythrocytesExerciseFemaleHemoglobinsHumansHypoxiaMaleMuscle, SkeletalNitric OxideNitritesOxygenHemoglobinsNitric OxideNitritesOxygenS-nitrosohemoglobinbrainhypoxia, exercisemusclesnitric oxidephysiology

Identifiers

PMID27881556
OpenAlexW2553395030

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.