ArticlePloS one2016
Inhibition of the TGFβ Pathway Enhances Retinal Regeneration in Adult Zebrafish.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 31 citations in OpenAlex.
- Review
- Automated In Vivo Phenotypic Screening Platform for Identifying Factors that Affect Cell Regeneration Kinetics.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Loss of the crumbs cell polarity complex disrupts epigenetic transcriptional control and cell cycle progression in the developing retina.The Journal of pathology · 2023Article
- Heterogeneity in quiescent Müller glia in the uninjured zebrafish retina drive differential responses following photoreceptor ablation.Frontiers in molecular neuroscience · 2023Article
- Vegf signaling between Müller glia and vascular endothelial cells is regulated by immune cells and stimulates retina regeneration.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Article
- Nuclear Factor I in neurons, glia and during the formation of Müller glia-derived progenitor cells in avian, porcine and primate retinas.The Journal of comparative neurology · 2022Article
- Nitroreductase/Metronidazole-Mediated Ablation and a MATLAB Platform (RpEGEN) for Studying Regeneration of the Zebrafish Retinal Pigment Epithelium.Journal of visualized experiments : JoVE · 2022Article
- Retina regeneration: lessons from vertebrates.Oxford open neuroscience · 2022Review
- Comparison of Glaucoma-Relevant Transcriptomic Datasets Identifies Novel Drug Targets for Retinal Ganglion Cell Neuroprotection.Journal of clinical medicine · 2021Article
- Müller Glia-Mediated Retinal Regeneration.Molecular neurobiology · 2021Review
- Review
- The complexity of TGFβ/activin signaling in regeneration.Journal of cell communication and signaling · 2021Review
- Involvement of TGFβ signaling pathway in oxidative stress and diabetic retinopathy.Archives of clinical and experimental ophthalmology · 2021Article
- Dpp/TGFβ-superfamily play a dual conserved role in mediating the damage response in the retina.PloS one · 2021Article
- Diverse Signaling by TGFβ Isoforms in Response to Focal Injury is Associated with Either Retinal Regeneration or Reactive Gliosis.Cellular and molecular neurobiology · 2021Article
- Tgfb3 collaborates with PP2A and notch signaling pathways to inhibit retina regeneration.eLife · 2020Article
- Biphasic Role of Tgf-β Signaling during Müller Glia Reprogramming and Retinal Regeneration in Zebrafish.iScience · 2020Article
- Involvement ofFrontiers in molecular biosciences · 2020Article
- Polygonatum sibiricum polysaccharide potentially attenuates diabetic retinal injury in a diabetic rat model.Journal of diabetes investigation · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In contrast to the mammalian retina, the zebrafish retina exhibits the potential for lifelong retinal neurogenesis and regeneration even after severe damage. Previous studies have shown that the transforming growth factor beta (TGFβ) signaling pathway is activated during the regeneration of different tissues in the zebrafish and is needed for regeneration in the heart and the fin. In this study, we have investigated the role of the TGFβ pathway in the N-methyl-N-nitrosourea (MNU)-induced chemical model of rod photoreceptor de- and regeneration in adult zebrafish. Immunohistochemical staining for phosphorylated Smad3 was elevated during retinal regeneration, and phosphorylated Smad3 co-localized with proliferating cell nuclear antigen and glutamine synthetase, indicating TGFβ pathway activation in proliferating Müller glia. Inhibiting the TGFβ signaling pathway using a small molecule inhibitor (SB431542) resulted in accelerated recovery from retinal degeneration. Accordingly, we observed increased cell proliferation in the outer nuclear layer at days 3 to 8 after MNU treatment. In contrast to the observations in the heart and the fin, the inhibition of the TGFβ signaling pathway resulted in increased proliferation after the induction of retinal degeneration. A better understanding of the underlying pathways with the possibility to boost retinal regeneration in adult zebrafish may potentially help to stimulate such proliferation also in other species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.