ArticlePloS one2016
From Lab to Lake - Evaluation of Current Molecular Methods for the Detection of Infectious Enteric Viruses in Complex Water Matrices in an Urban Area.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 48 citations in OpenAlex.
- Rapid differentiation of viable and inactivated African swine fever virus by a viability quantitative PCR.Veterinary research · 2025Article
- Enteroviruses in Water: Epidemiology, Detection and Inactivation.Environmental microbiology · 2025Review
- Evaluation of Three Viral Capsid Integrity qPCR Methods for Wastewater-Based Viral Surveillance.Food and environmental virology · 2025Article
- Rotavirus in Water Environments: A Systematic Review and Meta-Analysis.Environmental health insights · 2024Review
- Article
- The one health perspective to improve environmental surveillance of zoonotic viruses: lessons from COVID-19 and outlook beyond.ISME communications · 2022Review
- Capsid integrity quantitative PCR to determine virus infectivity in environmental and food applications - A systematic review.Water research X · 2021Article
- Occurrence of Human Enteric Viruses in Water Sources and Shellfish: A Focus on Africa.Food and environmental virology · 2021Review
- Rotavirus Surveillance in Tap Water, Recycled Water, and Sewage Sludge in Thailand: A Longitudinal Study, 2007-2018.Food and environmental virology · 2021Article
- Assessment of the Presence of Hepatitis E virus in Surface Water and Drinking Water in Portugal.Microorganisms · 2020Article
- The performance of biological and tertiary wastewater treatment procedures for rotaviruses A removal.Environmental science and pollution research international · 2020Article
- Assessing the Occurrence of Waterborne Viruses in Reuse Systems: Analytical Limits and Needs.Pathogens (Basel, Switzerland) · 2019Review
- Quantitative PCR-based identification of enteric viruses contaminating fresh produce and surface water used for irrigation in Egypt.Environmental science and pollution research international · 2019Article
- Quantification of human adenovirus and norovirus in river water in the north-east of France.Environmental science and pollution research international · 2018Article
- Prevalence of Rotavirus Genogroup A and Norovirus Genogroup II in Bassaseachic Falls National Park Surface Waters in Chihuahua, Mexico.International journal of environmental research and public health · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantitative PCR methods are commonly used to monitor enteric viruses in the aquatic environment because of their high sensitivity, short reaction times and relatively low operational cost. However, conclusions for public health drawn from results of such molecular techniques are limited due to their inability to determine viral infectivity. Ethidium monoazide (EMA) and propidium monoazide (PMA) are capable to penetrate the damaged or compromised capsid of the inactivated viruses and bind to the viral nucleic acids. We assessed whether dye treatment is a suitable approach to improve the ability of qPCR to distinguish between infectious and non-infectious human adenovirus, enterovirus and rotavirus A in surface water of an urban river and sewage before and after UV disinfection. Like the gold standard of cell culture assays, pretreatment EMA-/PMA-qPCR succeeded in removing false positive results which would lead to an overestimation of the viral load if only qPCR of the environmental samples was considered. A dye pretreatment could therefore provide a rapid and relatively inexpensive tool to improve the efficacy of molecular quantification methods in regards to viral infectivity.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.